Khan Junad, Ramadan Khaled, Korczeniewska Olga, Anwer Muhammad Moin, Benoliel Rafael, Eliav Eli
Department of Diagnostic Sciences, Rutgers School of Dental Medicine, NJ, United States.
Department of Diagnostic Sciences, Rutgers School of Dental Medicine, NJ, United States.
Neurosci Lett. 2015 Apr 10;592:99-106. doi: 10.1016/j.neulet.2015.03.001. Epub 2015 Mar 7.
Interleukin-10 (IL-10) is an anti-inflammatory cytokine that has been shown to play a role in inflammatory and autoimmune disorders as well as in neuropathic pain conditions. The objective of the present study was to assess the levels of IL-10 in rat's dorsal root ganglion (DRG) and the sciatic nerve following four different forms of sciatic nerve injury. The models used to induce the injury included two models of partial nerve injury: partial sciatic ligation (PSL) and chronic constriction injury (CCI), a model of complete sciatic transection (CST) and a model of perineural inflammation with minimal nerve damage (neuritis). Withdrawal responses for mechanical stimulus and withdrawal latency for thermal stimulation were used to measure mechanical and thermal hyperalgesia, respectively, and duration of the nociceptive withdrawal reflex to mechanical stimulus was used to measure mechanical hyperalgesia. The affected and contra-lateral nerves and the affected side DRG IL-10 levels were assessed by the means of enzyme-linked immunosorbent assay (ELISA), 3 and 8 days following the procedure and were compared to naïve rats' IL-10 levels. The rats exposed to CCI and neuritis developed significant mechanical and thermal hyperalgesia as well as mechanical hyperalgesia 3 and 8 days following the surgical procedure. Rats exposed to CST did not respond to mechanical stimulation and developed thermal hypoalgesia 3 and 8 days after the surgery. The DRG IL-10 levels were significantly reduced 3 and 8 days following CCI and PSL, significantly increased 3 and 8 days following CST, and remained unchanged following neuritis. The sciatic nerve IL-10 levels reduced significantly in both injured and contra-lateral nerves 3 and 8 days following CCI and PSL, elevated significantly in the injured but not in the contra-lateral nerve 3 and 8 days following CST and remained unchanged following neuritis. The results of this study suggest that IL-10's role in the neuropathic pain etiology may be specific to nerve injury type. Complete nerve transection increases while partial nerve injury reduces IL-10 levels in the involved nerve, and DRG. Perineural inflammation with minimal nerve damage has no effect on IL-10 levels.
白细胞介素 -10（IL -10）是一种抗炎细胞因子，已被证明在炎症和自身免疫性疾病以及神经性疼痛病症中发挥作用。本研究的目的是评估四种不同形式的坐骨神经损伤后大鼠背根神经节（DRG）和坐骨神经中IL -10的水平。用于诱导损伤的模型包括两种部分神经损伤模型：部分坐骨神经结扎（PSL）和慢性压迫损伤（CCI），一种完全坐骨神经横断（CST）模型以及一种神经损伤最小的神经周围炎症模型（神经炎）。分别使用对机械刺激的退缩反应和对热刺激的退缩潜伏期来测量机械性和热性痛觉过敏，并用对机械刺激的伤害性退缩反射持续时间来测量机械性痛觉过敏。在手术操作3天和8天后，通过酶联免疫吸附测定（ELISA）评估受影响侧和对侧神经以及受影响侧DRG的IL -10水平，并与未处理大鼠的IL -10水平进行比较。暴露于CCI和神经炎的大鼠在手术后3天和8天出现明显的机械性和热性痛觉过敏以及机械性痛觉过敏。暴露于CST的大鼠在手术后3天和8天对机械刺激无反应，并出现热感觉减退。在CCI和PSL后3天和8天，DRG的IL -10水平显著降低，在CST后3天和8天显著升高，而在神经炎后保持不变。在CCI和PSL后3天和8天，坐骨神经的IL -10水平在受伤侧和对侧神经中均显著降低，在CST后3天和8天，受伤侧神经中显著升高而对侧神经中未升高，在神经炎后保持不变。本研究结果表明，IL -10在神经性疼痛病因中的作用可能因神经损伤类型而异。完全性神经横断会使受累神经和DRG中的IL -10水平升高，而部分性神经损伤会使其降低。神经损伤最小的神经周围炎症对IL -10水平无影响。
