Ip Pui-Leng, Birnbaum Kenneth D
Department of Biology, Center for Genomics and Systems Biology, New York University, New York, NY, USA.
Methods Mol Biol. 2015;1284:175-83. doi: 10.1007/978-1-4939-2444-8_8.
Many cell populations have been labeled using stable reporters containing a fluorescent protein. These same marker lines can be used to capture specific cell types or marked cell populations after a brief enzymatic digestion to dissociate tissues. Here, we describe the use of Fluorescence Activated Cell Sorting (FACS) to isolate a rare population of cells marked with Green Fluorescent Protein (GFP). The FACS is a powerful selection tool that can be used to isolate high-quality RNA while selecting for fluorescence, cell size, and other properties.
许多细胞群体已通过含有荧光蛋白的稳定报告基因进行标记。在对组织进行短暂酶消化以使其解离后，这些相同的标记系可用于捕获特定细胞类型或标记细胞群体。在此，我们描述了使用荧光激活细胞分选（FACS）来分离用绿色荧光蛋白（GFP）标记的稀有细胞群体。FACS是一种强大的分选工具，可用于在选择荧光、细胞大小和其他特性的同时分离高质量RNA。
