Slane Daniel, Bayer Martin
Department of Cell Biology, Max Planck Institute for Developmental Biology, Spemannstrasse 35, 72076, Tuebingen, Germany.
Methods Mol Biol. 2017;1629:27-35. doi: 10.1007/978-1-4939-7125-1_3.
Fluorescence-activated cell sorting (FACS) is a powerful method for the analysis of cell type-specific transcriptome profiles, DNA or histone modifications, and chemical compounds. In plants, it has been employed mainly with root and shoot tissue in combination with cell wall digestion on cellular and nuclear content. However, many tissues are recalcitrant to cell separation and are therefore not readily accessible for FACS analysis. Here, we lay out a detailed protocol for the generation of transcriptional profiles from fluorescently labeled nuclei. The protocol described in this chapter has been used successfully to generate a transcriptional map of the early Arabidopsis thaliana embryo.
荧光激活细胞分选（FACS）是一种用于分析细胞类型特异性转录组图谱、DNA或组蛋白修饰以及化合物的强大方法。在植物中，它主要与根和茎组织结合使用，并对细胞和细胞核内容物进行细胞壁消化。然而，许多组织难以进行细胞分离，因此不容易用于FACS分析。在这里，我们制定了一个从荧光标记的细胞核生成转录图谱的详细方案。本章所述的方案已成功用于生成拟南芥早期胚胎的转录图谱。
