Jiang Lichun, Ruan Qiping, Chen Wei
a Key Laboratory for Molecular Biology and Biopharmaceutics , School of Life Science and Technology and.
b Ecological Security and Protection Key Laboratory of Sichuan Province , Mianyang Normal University , Mianyang , Sichuan , P.R. China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Sep;27(5):3184-5. doi: 10.3109/19401736.2015.1007327. Epub 2015 Mar 11.
The Xizang Plateau frog (Nanorana parkeri) belongs to the family Dicroglossidae, which distributes in southern and eastern Xizang, southern-most Qinghai in China, high elevations of north-central Nepal, Himalayan Bhutan, northeastern Kashmir and India. In this study, the complete mitochondrial genome of N. parkeri was sequenced. The mitogenome was 17,837 bp in length, consisting of 13 protein-coding genes, 22 transfer RNA (tRNA) genes, two ribosomal RNA genes, and a non-coding control region (CR). As in other vertebrates, most mitochondrial genes are encoded on the heavy strand, except for ND6 and eight tRNA genes, which are encoded on the light strand. The overall base composition of the N. parkeri is A: 27.7 % A, T: 30.1 % T, C: 26.6% and G: 15.6%. The alignment of the Nanorana species CRs exhibited high genetic variability and rich A + T content. In comparison with the mtDNA sequences typical of vertebrates, a tandem duplication of the tRNA(Met) gene and a rearrangement of the tRNA(Thr), tRNA(Pro) and tRNA(Leu) (CUN) genes were found. The complete mitogenome of N. parkeri can provided an important data for the studies on phylogenetic relationship and population genetics to further explore the taxonomic status of this species.
西藏高原蛙（Nanorana parkeri）属于叉舌蛙科，分布于中国西藏南部和东部、青海最南部、尼泊尔中北部高海拔地区、喜马拉雅地区的不丹、克什米尔东北部和印度。在本研究中，对西藏高原蛙的完整线粒体基因组进行了测序。该线粒体基因组长度为17,837 bp，由13个蛋白质编码基因、22个转运RNA（tRNA）基因、两个核糖体RNA基因和一个非编码控制区（CR）组成。与其他脊椎动物一样，大多数线粒体基因由重链编码，除了ND6和8个tRNA基因由轻链编码。西藏高原蛙的总体碱基组成为A：27.7%、T：30.1%、C：26.6%、G：15.6%。叉舌蛙属物种控制区的比对显示出高遗传变异性和丰富的A+T含量。与典型的脊椎动物线粒体DNA序列相比，发现了tRNA(Met)基因的串联重复以及tRNA(Thr)、tRNA(Pro)和tRNA(Leu)（CUN）基因的重排。西藏高原蛙的完整线粒体基因组可为系统发育关系和种群遗传学研究提供重要数据，以进一步探索该物种的分类地位。
