Ghebes Corina Adriana, Kelder Cindy, Schot Thomas, Renard Auke J, Pakvis Dean F M, Fernandes Hugo, Saris Daniel B
MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, The Netherlands.
Department of Orthopaedic Surgery, Medisch Spectrum Twente Hospital, Enschede, The Netherland.
J Tissue Eng Regen Med. 2017 Apr;11(4):1077-1088. doi: 10.1002/term.2009. Epub 2015 Mar 11.
Anterior cruciate ligament (ACL) reconstruction involves the replacement of the torn ligament with a new graft, often a hamstring tendon (HT). Described as similar, the ACL and HT have intrinsic differences related to their distinct anatomical locations. From a cellular perspective, identifying these differences represents a step forward in the search for new cues that enhance recovery after the reconstruction. The purpose of this study was to characterize the phenotype and multilineage potential of ACL- and HT-derived cells. ACL- and HT-derived cells were isolated from tissue harvest from patients undergoing total knee arthroplasty (TKA) or ACL reconstruction. In total, three ACL and three HT donors were investigated. Cell morphology, self-renewal potential (CFU-F), surface marker profiling, expression of tendon/ligament-related markers (PCR) and multilineage potential were analysed for both cell types; both had fibroblast-like morphology and low self-renewal potential. No differences in the expression of tendon/ligament-related genes or a selected set of surface markers were observed between the two cell types. However, differences in their multilineage potential were observed: while ACL-derived cells showed a high potential to differentiate into chondrocytes and adipocytes, but not osteoblasts, HT-derived cells showed poor potential to form adipocytes, chondrocytes and osteoblasts. Our results demonstrated that HT-derived cells have low multilineage potential compared to ACL-derived cells, further highlighting the need for extrinsic signals to fully restore the function of the ACL upon reconstruction. Copyright © 2015 John Wiley & Sons, Ltd.
前交叉韧带（ACL）重建术涉及用新的移植物替换撕裂的韧带，常用的移植物是腘绳肌腱（HT）。尽管ACL和HT被描述为相似，但它们因其不同的解剖位置而存在内在差异。从细胞层面来看，识别这些差异是在寻找促进重建后恢复的新线索方面向前迈出的一步。本研究的目的是对ACL来源的细胞和HT来源的细胞的表型及多向分化潜能进行表征。ACL来源的细胞和HT来源的细胞是从接受全膝关节置换术（TKA）或ACL重建术的患者的组织样本中分离出来的。总共对3名ACL供体和3名HT供体进行了研究。对这两种细胞类型的细胞形态、自我更新潜能（集落形成单位 - 成纤维细胞，CFU - F）、表面标志物分析、肌腱/韧带相关标志物的表达（PCR）以及多向分化潜能进行了分析；二者均具有成纤维细胞样形态且自我更新潜能较低。在这两种细胞类型之间，未观察到肌腱/韧带相关基因或一组选定表面标志物的表达存在差异。然而，观察到了它们在多向分化潜能方面的差异：虽然ACL来源的细胞具有向软骨细胞和脂肪细胞分化的高潜能，但向成骨细胞分化的潜能较低，而HT来源的细胞形成脂肪细胞、软骨细胞和成骨细胞的潜能较差。我们的结果表明，与ACL来源的细胞相比，HT来源的细胞多向分化潜能较低，这进一步凸显了在重建时需要外部信号来完全恢复ACL功能的必要性。版权所有© 2015约翰威立父子有限公司。
