Taylor Linnéa, Arnér Karin, Engelsberg Karl, Ghosh Fredrik
Department of Ophthalmology, Lund University Hospital, Lund, Sweden.
Department of Ophthalmology, Lund University Hospital, Lund, Sweden.
Int J Dev Neurosci. 2015 May;42:46-58. doi: 10.1016/j.ijdevneu.2015.03.002. Epub 2015 Mar 7.
To examine the expression of interstitial extracellular matrix components and their role during retinal development.
Fibronectin (FN), collagen IV (Coll IV) and laminin 5 (Lam 5) expression in rat retinas from developmental stages E17 to adult were studied. In addition, PN5 full-thickness retinas were cultured for 7 days with dispase, which selectively cleaves FN and Coll IV, at either 0.5 U/ml or 5.0 U/ml for 3 or 24h. Eyecups and retinal cultures were examined morphologically using hematoxylin and eosin staining and immunohistochemistry.
Coll IV, Lam 5 and FN were all transiently expressed in the interstitial matrix of the retinal layers during development. The retinal layers in dispase treated explants was severely disturbed in a dose and time dependent manner.
FN, Lam 5 and Coll IV, are present in the interstitial extracellular matrix during rat retinal development. Enzymatic cleavage of FN and Coll IV early in the lamination process disrupts the retinal layers implicating their pivotal role in this process.
研究视网膜发育过程中间质细胞外基质成分的表达及其作用。
研究了从胚胎第17天到成年大鼠视网膜中纤连蛋白（FN）、IV型胶原（Coll IV）和层粘连蛋白5（Lam 5）的表达。此外，将出生后第5天的全层视网膜用分散酶培养7天，分散酶可选择性切割FN和Coll IV，浓度分别为0.5 U/ml或5.0 U/ml，作用3小时或24小时。使用苏木精和伊红染色及免疫组织化学方法对眼球杯和视网膜培养物进行形态学检查。
在发育过程中，Coll IV、Lam 5和FN均在视网膜各层的间质基质中短暂表达。经分散酶处理的外植体中的视网膜各层受到严重干扰，且呈剂量和时间依赖性。
在大鼠视网膜发育过程中，FN、Lam 5和Coll IV存在于间质细胞外基质中。在视网膜分层过程早期对FN和Coll IV进行酶切会破坏视网膜各层，这表明它们在该过程中起关键作用。
