Shah Pratik, Choi Suk Won, Kim Ho-jin, Cho Seok Keun, Thulstrup Peter Waaben, Bjerrum Morten Jannik, Bhang Yong-Joo, Ahn Jong Cheol, Yang Seong Wook
UNIK Center for Synthetic Biology, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Copenhagen, Denmark.
Analyst. 2015 May 21;140(10):3422-30. doi: 10.1039/c5an00093a. Epub 2015 Mar 11.
In recent years microRNAs (miRNAs) have been established as important biomarkers in a variety of diseases including cancer, diabetes, cardiovascular disease, aging, Alzheimer's disease, asthma, autoimmune disease and liver diseases. As a consequence, a variety of monitoring methods for miRNAs have been developed, including a fast and simple method for miRNA detection by exploitation of the unique photoluminescence of DNA-templated silver nanoclusters (DNA/AgNCs). To increase the versatility of the AgNC-based method, we have adopted DNA/RNA chimera templates for AgNC-based probes, allowing response from several human miRNAs which are hardly detectable with DNA-based probes. Here, we demonstrate in detail the power of DNA/RNA chimera/AgNC probes in detecting two human miRNAs, let-7a and miR-200c. The DNA/RNA chimera-based probes are highly efficient to determine the level of miRNAs in several human cell lines.
近年来，微小RNA（miRNA）已被确立为多种疾病的重要生物标志物，这些疾病包括癌症、糖尿病、心血管疾病、衰老、阿尔茨海默病、哮喘、自身免疫性疾病和肝脏疾病。因此，已经开发了多种用于miRNA的监测方法，包括一种利用DNA模板化银纳米簇（DNA/AgNCs）独特的光致发光进行miRNA检测的快速简便方法。为了提高基于AgNCs方法的通用性，我们采用了基于DNA/RNA嵌合体模板的AgNCs探针，使得几种难以用基于DNA的探针检测到的人类miRNA能够产生响应。在此，我们详细展示了DNA/RNA嵌合体/AgNCs探针在检测两种人类miRNA（let-7a和miR-200c）方面的能力。基于DNA/RNA嵌合体的探针在确定几种人类细胞系中miRNA的水平方面非常高效。
