Department of Applied Chemistry, Graduate School of Kanagawa Institute of Technology, 1030 Shimoogino, Atsugi-shi, Kanagawa 243-0292, Japan ; Department of Nutrition and Life Science, Kanagawa Institute of Technology, 1030 Shimoogino, Atsugi-shi, Kanagawa 243-0292, Japan.
Department of Nutrition and Life Science, Kanagawa Institute of Technology, 1030 Shimoogino, Atsugi-shi, Kanagawa 243-0292, Japan.
J Clin Biochem Nutr. 2015 Mar;56(2):143-8. doi: 10.3164/jcbn.14-107. Epub 2015 Jan 20.
Vitamin E analog, such as α- and γ-tocopherol, can undergo ω-oxidation without cleavage of the chroman ring, and this pathway is responsible for generation of the major urinary vitamin E metabolite, carboxyethyl hydroxychroman. However, it is still unclear how carboxyethyl hydroxychroman is changed in various tissues after vitamin E intake. We therefore investigated changes in the concentrations of α- and γ-tocopherol and their metabolites in rat liver and kidney. The concentration of α-tocopherol in rat liver increased until 6 h after oral administration, and then decreased. The change in the concentration of α-carboxyethyl hydroxychroman in rat liver in the α-Toc group slowly increased until 12 h after oral administration. Cytochrome P450 3A1 mRNA expression significantly increased from 12 h after the start of α-tocopherol administration. The change in the concentration of γ-carboxyethyl hydroxychroman in rat liver in the γ-Toc group markedly increased until 12 h after oral administration. On the other hand, γ-carboxyethyl hydroxychroman in rat kidney showed greater accumulation than α-carboxyethyl hydroxychroman from 3 h to 24 h after oral administration. From these results, we considered that γ-carboxyethyl hydroxychroman formed in the liver continues to be released into the bloodstream and is transported to the kidney rapidly.
维生素 E 类似物，如α-和γ-生育酚，可以在不裂解色满环的情况下进行 ω-氧化，该途径负责生成主要的尿维生素 E 代谢物，羧乙基羟色满。然而，维生素 E 摄入后羧乙基羟色满在各种组织中的变化方式仍不清楚。因此，我们研究了α-和γ-生育酚及其代谢物在大鼠肝脏和肾脏中的浓度变化。α-生育酚在大鼠肝脏中的浓度在口服后 6 小时内增加，然后减少。α-Toc 组大鼠肝脏中α-羧乙基羟色满的浓度变化在口服后 12 小时缓慢增加。从α-生育酚给药开始 12 小时后，细胞色素 P450 3A1 mRNA 表达显著增加。γ-Toc 组大鼠肝脏中γ-羧乙基羟色满的浓度变化在口服后 12 小时内明显增加。另一方面，口服后 3 小时至 24 小时，大鼠肾脏中的γ-羧乙基羟色满比α-羧乙基羟色满积累更多。从这些结果可以认为，在肝脏中形成的γ-羧乙基羟色满继续释放到血液中，并迅速被运送到肾脏。
