人血清中α-生育酚和γ-生育酚代谢产物2,5,7,8-四甲基-2-(2'-羧乙基)-6-羟基苯并二氢吡喃和2,7,8-三甲基-2-(2'-羧乙基)-6-羟基苯并二氢吡喃的定量分析。
Quantification of the alpha- and gamma-tocopherol metabolites 2,5,7, 8-tetramethyl-2-(2'-carboxyethyl)-6-hydroxychroman and 2,7, 8-trimethyl-2-(2'-carboxyethyl)-6-hydroxychroman in human serum.
作者信息
Stahl W, Graf P, Brigelius-Flohé R, Wechter W, Sies H
机构信息
Institut für Physiologische Chemie I, Heinrich-Heine-Universität Düsseldorf, Germany.
出版信息
Anal Biochem. 1999 Nov 15;275(2):254-9. doi: 10.1006/abio.1999.4312.
alpha- and gamma-tocopherol are the major vitamin E compounds found in human blood and tissues. The metabolites are 2,5,7, 8-tetramethyl-2-(2'-carboxyethyl)-6-hydroxychroman (alpha-CEHC) and 2,7,8-trimethyl-2-(2'-carboxyethyl)-6-hydroxychroman (gamma-CEHC, LLU-alpha), respectively. alpha-CEHC is excreted mainly as glucuronide or sulfate conjugates in the urine. Here we describe a sensitive and reliable method to analyze alpha- and gamma-CEHC in human serum. The concentration of alpha-CEHC in human serum is in the range of 5-10 pmol/ml but increases significantly up to 200 pmol/ml upon supplementation with RRR-alpha-tocopherol. About one-third of the alpha-CEHC circulating in the blood is present as a glucuronide conjugate. Baseline levels of gamma-CEHC are about 50 to 85 pmol/ml.
α-生育酚和γ-生育酚是在人体血液和组织中发现的主要维生素E化合物。其代谢产物分别是2,5,7,8-四甲基-2-(2'-羧乙基)-6-羟基色满（α-CEHC）和2,7,8-三甲基-2-(2'-羧乙基)-6-羟基色满（γ-CEHC，LLU-α）。α-CEHC主要以葡萄糖醛酸苷或硫酸酯共轭物的形式经尿液排出。在此我们描述了一种灵敏且可靠的方法来分析人血清中的α-CEHC和γ-CEHC。人血清中α-CEHC的浓度在5-10 pmol/ml范围内，但在补充RRR-α-生育酚后会显著升高至200 pmol/ml。血液中循环的α-CEHC约有三分之一以葡萄糖醛酸苷共轭物的形式存在。γ-CEHC的基线水平约为50至85 pmol/ml。
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