†Department of Chemistry, University of Toronto, 80 St George Street, Toronto, Ontario M5S 3H6, Canada.
‡Institute of Biomaterials and Biomedical Engineering, 164 College Street, Toronto, Ontario M5S 3G9, Canada.
Anal Chem. 2015 Apr 7;87(7):3902-10. doi: 10.1021/ac504697r. Epub 2015 Mar 11.
There is great interest in the development of integrated tools allowing for miniaturized sample processing, including solid phase extraction (SPE). We introduce a new format for microfluidic SPE relying on C18-functionalized magnetic beads that can be manipulated in droplets in a digital microfluidic platform. This format provides the opportunity to tune the amount (and potentially the type) of stationary phase on-the-fly, and allows the removal of beads after the extraction (to enable other operations in same device-space), maintaining device reconfigurability. Using the new method, we employed a design of experiments (DOE) operation to enable automated on-chip optimization of elution solvent composition for reversed phase SPE of a model system. Further, conditions were selected to enable on-chip fractionation of multiple analytes. Finally, the method was demonstrated to be useful for online cleanup of extracts from dried blood spot (DBS) samples. We anticipate this combination of features will prove useful for separating a wide range of analytes, from small molecules to peptides, from complex matrices.
人们对开发集成工具以实现小型化样品处理（包括固相萃取（SPE））非常感兴趣。我们引入了一种新的微流控 SPE 格式，该格式依赖于可以在数字微流控平台中的液滴中操纵的 C18 功能化磁性珠。这种格式提供了在飞行中调整固定相的量（和潜在类型）的机会，并允许在提取后去除珠（以在同一设备空间中进行其他操作），保持设备的可重构性。使用新方法，我们采用实验设计（DOE）操作，以实现模型系统反相 SPE 洗脱溶剂组成的自动芯片优化。此外，还选择了条件以实现多种分析物的芯片内馏分。最后，该方法被证明可用于在线净化干血斑（DBS）样品提取物。我们预计这种组合功能将有助于从复杂基质中分离广泛的分析物，从小分子到肽。
