University of Calgary, 3280 Hospital Drive NW, Calgary, Alberta T2N 4Z6, Canada.
University of Calgary, 2500 University Drive NW, Calgary, Alberta T2N 1N4, Canada.
Bone Joint Res. 2015 Mar;4(3):38-44. doi: 10.1302/2046-3758.43.2000339.
Ligaments which heal spontaneously have a healing process that is similar to skin wound healing. Menopause impairs skin wound healing and may likewise impair ligament healing. Our purpose in this study was to investigate the effect of surgical menopause on ligament healing in a rabbit medial collateral ligament model.
Surgical menopause was induced with ovariohysterectomy surgery in adult female rabbits. Ligament injury was created by making a surgical gap in the midsubstance of the medial collateral ligament. Ligaments were allowed to heal for six or 14 weeks in the presence or absence of oestrogen before being compared with uninjured ligaments. Molecular assessment examined the messenger ribonucleic acid levels for collagens, proteoglycans, proteinases, hormone receptors, growth factors and inflammatory mediators. Mechanical assessments examined ligament laxity, total creep strain and failure stress.
Surgical menopause in normal medial collateral ligaments initiated molecular changes in all the categories evaluated. In early healing medial collateral ligaments, surgical menopause resulted in downregulation of specific collagens, proteinases and inflammatory mediators at 6 weeks of healing, and proteoglycans, growth factors and hormone receptors at 14 weeks of healing. Surgical menopause did not produce mechanical changes in normal or early healing medial collateral ligaments. With or without surgical menopause, healing ligaments exhibited increased total creep strain and decreased failure stress compared with uninjured ligaments.
Surgical menopause did not affect the mechanical properties of normal or early healing medial collateral ligaments in a rabbit model. The results in this preclinical model suggest that menopause may result in no further impairment to the ligament healing process. Cite this article: Bone Joint Res 2015;4:38-44.
能够自行愈合的韧带的愈合过程与皮肤创伤愈合相似。绝经会损害皮肤创伤愈合，同样可能会损害韧带愈合。本研究旨在通过兔内侧副韧带模型研究手术绝经对韧带愈合的影响。
通过卵巢切除术对成年雌性兔进行手术绝经。通过在中内侧副韧带的实质部位制造手术间隙来造成韧带损伤。在有或没有雌激素的情况下，让韧带愈合 6 或 14 周，然后与未受伤的韧带进行比较。分子评估检查了胶原蛋白、蛋白聚糖、蛋白酶、激素受体、生长因子和炎症介质的信使核糖核酸水平。力学评估检查了韧带松弛度、总蠕变量和断裂应力。
正常内侧副韧带的手术绝经引发了所有评估类别的分子变化。在早期愈合的内侧副韧带中，手术绝经导致特定胶原蛋白、蛋白酶和炎症介质在愈合 6 周时下调，以及蛋白聚糖、生长因子和激素受体在愈合 14 周时下调。手术绝经未在正常或早期愈合的内侧副韧带中产生力学变化。无论是否进行手术绝经，愈合的韧带与未受伤的韧带相比，总蠕变量增加，断裂应力降低。
手术绝经不会影响兔模型中正常或早期愈合的内侧副韧带的力学特性。该临床前模型的结果表明，绝经可能不会对韧带愈合过程造成进一步的损害。
Bone Joint Res 2015;4:38-44.
