Diekmann Ulf, Naujok Ortwin
Institute of Clinical Biochemistry, Hannover Medical School, 30625, Hannover, Germany.
Methods Mol Biol. 2016;1341:157-72. doi: 10.1007/7651_2015_220.
Differentiation of pluripotent stem cells into cells of the definitive endoderm requires an in vitro gastrulation event. Differentiated somatic cells derived from this germ layer may then be used for cell replacement therapies of degenerative diseases of the liver, lung, and pancreas. Here we describe an endoderm differentiation protocol, which initiates the differentiation from a defined cell number of dispersed single cells and reliably yields in >70-80 % endoderm-committed cells in a short 5-day treatment regimen.
将多能干细胞分化为定形内胚层细胞需要体外原肠胚形成事件。源自该胚层的分化体细胞随后可用于肝脏、肺和胰腺退行性疾病的细胞替代疗法。在此，我们描述了一种内胚层分化方案，该方案从确定数量的分散单细胞开始分化，并在短短5天的治疗方案中可靠地产生>70-80%的内胚层定向细胞。
