Rulík Martin, Chaudhary Prem Prashant
Laboratory of Aquatic Microbial Ecology Department of Ecology and Environmental Sciences Faculty of Science Palacky University Olomouc Czech Republic Laboratory of Aquatic Microbial Ecology, Department of Ecology and Environmental Sciences, Faculty of Science, Palacky University, Olomouc, Czech Republic.
Braz J Microbiol. 2015 Mar 4;45(4):1255-61. doi: 10.1590/s1517-83822014000400016. eCollection 2014.
Magnetotactic bacteria (MTB) are of considerable interest because of their importance in the manufacture of various bioinspired materials. In order to find out the status of magnetotactic bacteria at three different sediment in Czech Republic, samples collected from both standing and running freshwaters were subjected to molecular diversity analysis by using 16S rRNA gene approach. Total community DNA from sediment sample was isolated and used for PCR, cloning and sequence analysis. Of the 24 analyzed sequences, six clones are closely related to Magnetobacterium sp. affiliated with the Nitrospira phylum which showed the dominance of Magnetobacterium phylotypes in the sample. This study will provide useful insight about the community structure of MTB in this particular geographical region. However more detailed and specific studies are warranted in order to properly assess the community structure of MTB's in fresh water sediments.
趋磁细菌(MTB)因其在制造各种仿生材料方面的重要性而备受关注。为了了解捷克共和国三种不同沉积物中趋磁细菌的状况,采用16S rRNA基因方法对从静水和流水淡水中采集的样本进行了分子多样性分析。从沉积物样本中分离出总群落DNA,并用于PCR、克隆和序列分析。在分析的24个序列中,有6个克隆与硝化螺旋菌门的趋磁杆菌属密切相关,这表明样本中趋磁杆菌属的系统型占主导地位。这项研究将为该特定地理区域内趋磁细菌的群落结构提供有用的见解。然而,为了正确评估淡水沉积物中趋磁细菌的群落结构,还需要进行更详细和具体的研究。