从临床样本分离的金黄色葡萄球菌中I类整合子的检测
Detection of Class I Integrons in Staphyloacoccus aurous Isolated From Clinical Samples.
作者信息
Yahaghi Emad, Imani Fooladi Abbas Ali, Amin Mohsen, Mirnejad Reza, Nezamzade Reza, Amani Jafar
机构信息
Department of Microbiology, Science and Research Branch, Islamic Azad University, Damghan, IR Iran.
Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran.
出版信息
Iran Red Crescent Med J. 2014 Nov 10;16(11):e16234. doi: 10.5812/ircmj.16234. eCollection 2014 Nov.
BACKGROUND
Staphylococcus aurous is a major pathogen, causing variety of diseases and death in Iran and in the world. Despite the use of a spectrum of new antibiotics, this organism has caused severe infections in burns as well as in different parts of the body, due to acquired drug resistance. Widespread inappropriate use of antibiotics in treating bacterial infections has led to the selection and circulation of resistant strains and the growing risk of transferring resistant genes to sensitive bacteria. One of the causes of antibiotic resistance in S. aurous strains is the gain of resistance genes including integrase and qac/sul1.
OBJECTIVES
The purpose of this study was to investigate the presence of class 1 integron in S. aurous strains isolated from clinical samples for the first time in Iran.
MATERIALS AND METHODS
This descriptive study was performed on 200 strains of S. aurous isolated from patients admitted to Baqiyatallah Hospital in Tehran in 2013. These strains were confirmed using biochemical and serological tests and the presence of class 1 integron was determined by polymerase chain reaction (PCR).
RESULTS
Among the 200 samples, 1% of the strains (two isolates) contained the class 1 integron gene. The results of this study showed that the highest frequency of the obtained samples belonged to males and the isolates occurred mostly in individuals aged 51-60 years old. The highest number of strains was found in wound samples. The strains were most frequently isolated from the emergency ward and the intensive care unit (ICU).
CONCLUSIONS
Findings of this study showed that integron can have a limited frequency in S. aurous isolated from clinical sample in Tehran.
背景
金黄色葡萄球菌是一种主要病原体，在伊朗乃至全世界都可引发多种疾病并导致死亡。尽管使用了一系列新型抗生素，但由于获得性耐药，这种微生物在烧伤患者以及身体不同部位仍会引发严重感染。在治疗细菌感染过程中广泛且不恰当使用抗生素，导致耐药菌株的选择与传播，以及耐药基因向敏感细菌转移的风险不断增加。金黄色葡萄球菌菌株耐药的原因之一是获得了包括整合酶和qac/sul1在内的耐药基因。
目的
本研究旨在首次在伊朗调查从临床样本中分离出的金黄色葡萄球菌菌株中1类整合子的存在情况。
材料与方法
本描述性研究对2013年从德黑兰巴奇亚塔拉医院收治患者中分离出的200株金黄色葡萄球菌进行。这些菌株通过生化和血清学检测得以确认，1类整合子的存在通过聚合酶链反应（PCR）来确定。
结果
在200个样本中，1%的菌株（两株分离株）含有1类整合子基因。本研究结果表明，所获样本中男性的频率最高，分离株大多出现在51至60岁的个体中。伤口样本中的菌株数量最多。这些菌株最常从急诊科和重症监护病房（ICU）分离得到。
结论
本研究结果表明，在从德黑兰临床样本中分离出的金黄色葡萄球菌中，整合子的频率可能有限。
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