Guo Wei, Wang Chengcheng, Zuo Zhangli, Qiu Jin-Long
a State Key Laboratory of Plant Genomics; Institute of Microbiology; Chinese Academy of Sciences; Beijing, PR China.
Plant Signal Behav. 2014;9(7):e29230. doi: 10.4161/psb.29230.
Anion efflux is one of the most immediate responses of plant cells to pathogen attacks, suggesting that anion channels may play a role in plant defense. Recently we reported that the chloride channel AtCLCd negatively regulates Arabidopsis pathogen-associated molecular pattern-triggered immunity (PTI), probably by affecting trafficking of the pattern recognition receptors (PRRs). Since AtCLCd is localized to the trans-Golgi network, it is not likely to be directly involved in anion flux across the plasma membrane. Here, we used a pharmacological approach to explore further the function of plasma membrane-localized R-type and S-type anion channels in plant immunity. We found that the R-type and S-type anion channels play opposite roles in Arabidopsis innate immunity. Inhibition of the R-type anion channels enhances, whereas inhibition of the S-type channels inhibits PTI and effector-triggered immunity (ETI).
阴离子外流是植物细胞对病原体攻击最直接的反应之一，这表明阴离子通道可能在植物防御中发挥作用。最近我们报道，氯离子通道AtCLCd负向调节拟南芥病原体相关分子模式触发的免疫反应（PTI），可能是通过影响模式识别受体（PRR）的运输。由于AtCLCd定位于反式高尔基体网络，它不太可能直接参与阴离子跨质膜的流动。在这里，我们使用药理学方法进一步探索质膜定位的R型和S型阴离子通道在植物免疫中的功能。我们发现，R型和S型阴离子通道在拟南芥固有免疫中发挥相反的作用。抑制R型阴离子通道会增强PTI，而抑制S型通道则会抑制PTI和效应子触发的免疫反应（ETI）。
