Qiu Xiuzhen, Liang Yong, Guo Huishi
Se Pu. 2014 Nov;32(11):1214-8. doi: 10.3724/sp.j.1123.2014.07001.
An effective method for the preparation of magnetic molecularly imprinted polymers (MIPs) on attapulgite (ATP) using microcystin-LR (MC-LR) as template molecule through reversible addition fragmentation chain transfer (RAFT) radical polymerization was reported. A novel MIPs stir bar sorptive extraction (SBSE) coating was prepared by sol-gel method. The structure and morphology of MIPs were characterized by infrared spectroscopy (IR) and transmission electron microscopy (TEM). The adsorption performance of SBSE coated with MC-LR was studied by high performance liquid chromatography (HPLC). The results showed that under the optimized experimental conditions, the present method has high selectivity to MC-LR. A good linearity was achieved for MC-LR over the range of 0.010-5.0 mg/L and the limit of detection (S/N = 3) was found to be as low as 0.27 μg/L. The proposed method was successfully applied to the determination of MC-LR in water samples. The average recoveries ranged from 83.33% to 100.07% with the relative standard deviations (RSDs) of 1.40%-9.17% at three spiked levels (20, 40 and 80 μg/L). The developed method is rapid, selective and sensitive, and adapts to the analysis of trace MC in water samples.
报道了一种通过可逆加成-断裂链转移（RAFT）自由基聚合，以微囊藻毒素-LR（MC-LR）为模板分子，在凹凸棒石（ATP）上制备磁性分子印迹聚合物（MIPs）的有效方法。采用溶胶-凝胶法制备了一种新型的MIPs搅拌棒吸附萃取（SBSE）涂层。通过红外光谱（IR）和透射电子显微镜（TEM）对MIPs的结构和形貌进行了表征。采用高效液相色谱（HPLC）研究了涂覆MC-LR的SBSE的吸附性能。结果表明，在优化的实验条件下，该方法对MC-LR具有高选择性。MC-LR在0.010 - 5.0 mg/L范围内具有良好的线性关系，检测限（S/N = 3）低至0.27 μg/L。该方法成功应用于水样中MC-LR的测定。在三个加标水平（20、40和80 μg/L）下，平均回收率为83.33% - 100.07%，相对标准偏差（RSDs）为1.40% - 9.17%。所建立的方法快速、选择性好、灵敏度高，适用于水样中痕量MC的分析。
