Jesus Desyree Murta, Moussatche Nissin, McFadden Baron B D, Nielsen Casey Paulasue, D'Costa Susan M, Condit Richard C
Department of Molecular Genetics and Microbiology, University of Florida, Gainesville, FL 32610, USA.
Department of Molecular Genetics and Microbiology, University of Florida, Gainesville, FL 32610, USA.
Virology. 2015 Jul;481:1-12. doi: 10.1016/j.virol.2015.02.020. Epub 2015 Mar 9.
Maturation of the vaccinia virion is an intricate process that results in the organization of the viroplasm contained in immature virions into the lateral bodies, core wall and nucleocapsid observed in the mature particles. It is unclear how this organization takes place and studies with mutants are indispensable in understanding this process. By characterizing an inducible mutant in the A3L gene, we revealed that A3, an inner core wall protein, is important for formation of normal immature viruses and also for the correct localization of L4, a nucleocapsid protein. L4 did not accumulate in the viral factories in the absence of A3 and was not encapsidated in the particles that do not contain A3. These data strengthen our previously suggested hypothesis that A3 and L4 interact and that this interaction is critical for proper formation of the core wall and nucleocapsid.
痘苗病毒粒子的成熟是一个复杂的过程，它导致未成熟病毒粒子中所含的病毒质被组织成在成熟粒子中观察到的侧体、核心壁和核衣壳。目前尚不清楚这种组织是如何发生的，利用突变体进行研究对于理解这一过程是必不可少的。通过对A3L基因中的一个可诱导突变体进行表征，我们发现A3是一种内核壁蛋白，对于正常未成熟病毒的形成以及核衣壳蛋白L4的正确定位都很重要。在没有A3的情况下，L4不会在病毒工厂中积累，也不会被包裹在不含A3的粒子中。这些数据强化了我们之前提出的假说，即A3和L4相互作用，并且这种相互作用对于核心壁和核衣壳的正确形成至关重要。
