Matsumoto Goichi, Ueda Takayo, Sugita Yoshihiko, Kubo Katsutoshi, Mizoguchi Megumi, Kotani Eiji, Oda Naoki, Kawamata Shin, Segami Natsuki, Mori Hajime
Division of Oral Surgery, Yokohama Clinical Education Center of Kanagawa Dental University, Kanagawa-ku, Yokohama, Japan Department of Oral and Maxillofacial Surgery, Kanazawa Medical University, Uchinada, Ishikawa, Japan
Insect Biomedical Research Center, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, Japan.
J Biomater Appl. 2015 Aug;30(2):193-200. doi: 10.1177/0885328215575763. Epub 2015 Mar 11.
Atelocollagen sponges incorporating polyhedra encapsulating bone morphogenetic protein 2 (BMP-2) were implanted into lateral bone defects in the mandible. Half of the bone defects on the left side were treated with atelocollagen sponges containing 1.8 × 10(7) BMP-2 polyhedra, and half were treated with sponges containing 3.6 × 10(6) BMP-2 polyhedra. As controls, we treated the right-side bone defects in each animal with an atelocollagen sponge containing 5 µg of recombinant human BMP-2 (rhBMP-2) or 1.8 × 10(7) empty polyhedral. After a healing period of six months, whole mandibles were removed for micro-computed tomography (CT) and histological analyses. Micro-CT images showed that more bone had formed at all experimental sites than at control sites. However, the density of the new bone was not significantly higher at sites with an atelocollagen sponge containing BMP-2 polyhedra than at sites with an atelocollagen sponge containing rhBMP-2 or empty polyhedra. Histological examination confirmed that the BMP-2 polyhedra almost entirely replaced the atelocollagen sponges and connected the original bone with the regenerated bone. These results show that the BMP-2 delivery system facilitates the regeneration of new bone in the mandibular alveolar bone ridge and has an advance in the technology of bone regeneration for implant site development.
将包裹有骨形态发生蛋白2（BMP - 2）的多面体的去端胶原蛋白海绵植入下颌骨的外侧骨缺损处。左侧一半的骨缺损用含有1.8×10⁷个BMP - 2多面体的去端胶原蛋白海绵治疗，另一半用含有3.6×10⁶个BMP - 2多面体的海绵治疗。作为对照，我们用含有5μg重组人BMP - 2（rhBMP - 2）的去端胶原蛋白海绵或1.8×10⁷个空多面体治疗每只动物右侧的骨缺损。经过六个月的愈合期后，取出整个下颌骨进行微型计算机断层扫描（CT）和组织学分析。微型CT图像显示，所有实验部位形成的骨量均多于对照部位。然而，含有BMP - 2多面体的去端胶原蛋白海绵部位的新骨密度并不显著高于含有rhBMP - 2或空多面体的去端胶原蛋白海绵部位。组织学检查证实，BMP - 2多面体几乎完全取代了去端胶原蛋白海绵，并将原始骨与再生骨连接起来。这些结果表明，BMP - 2递送系统促进了下颌牙槽骨嵴新骨的再生，并在种植位点开发的骨再生技术方面取得了进展。
