Yokoi T, Sagisaka K, Nakahori Y, Nakagome Y
Jinrui Idengaku Zasshi. 1989 Sep;34(3):209-15. doi: 10.1007/BF01900723.
A recombinant DNA probe (pHY10) hybridizing specifically to human DNA family DYZ1, 3,000 copies of which are present on the long arm of the Y chromosome, was used for probing human genome DNA digested with various restriction enzymes. To our surprise, the probe detected a hypervariable polymorphism of autosomal origin in human DNA when digested with TaqI. None of other 12 restriction enzymes revealed polymorphic patterns. Codominant segregation of the polymorphism was established in family studies. This probe has been widely used in the detection of the Y chromosome. Its ease of availability as well as highly discriminating polymorphic pattern makes it potentially very useful for forensic and human genetic purposes.
一种重组DNA探针（pHY10），它能与人类DNA家族DYZ1特异性杂交，在Y染色体长臂上有3000个拷贝，被用于探测经各种限制性酶消化的人类基因组DNA。令我们惊讶的是，当用TaqI消化时，该探针在人类DNA中检测到了常染色体来源的高变多态性。其他12种限制性酶均未显示出多态性模式。在家族研究中确定了该多态性的共显性分离。这种探针已被广泛用于Y染色体的检测。它易于获得以及高度区分的多态性模式使其在法医和人类遗传学目的方面具有潜在的非常有用的价值。
