Nanyang Environment & Water Research Institute, Advanced Environmental Biotechnology Centre, Nanyang Technological University, 637141, Singapore.
School of Materials Science & Engineering, College of Engineering, Nanyang Technological University, 637141, Singapore.
Water Res. 2015 May 15;75:123-30. doi: 10.1016/j.watres.2015.02.035. Epub 2015 Feb 28.
A rapid fluorescence measurement based on resazurin reduction was developed and applied for the detection of toxicants/inhibitors to anaerobic digestion metabolism. By initially using a pure facultative anaerobic strain, Enterococcus faecalis as a model organism, this technique proved to be fast and sensitive when detecting the model toxicant, pentachlorophenol (PCP). The technique revealed significant metabolic changes in Enterococcus faecalis with a PCP spike ranging from 0.05 to 100 mg/L, and could detect PCP's toxicity to E. faecalis at a concentration of only 0.05 mg/L in 8 min. Furthermore, by extending this technique to a mixed anaerobic sludge, not only could the effect of 0.05-100 mg/L PCP be determined on anaerobic digestion metabolism within 10 min, but also its rate of biogas production. These results suggest that a resazurin-based fluorescence measurement can potentially be incorporated into a microfluidic system to develop a biosensor for the real-time monitoring, control and early warning of toxicant/inhibitor loads in the influent to an anaerobic digestion system.
建立了一种基于 Resazurin 还原的快速荧光测量法，并将其应用于检测厌氧消化代谢的毒物/抑制剂。最初使用纯兼性厌氧菌株粪肠球菌作为模型生物，该技术在检测模型毒物五氯苯酚（PCP）时被证明是快速和灵敏的。该技术揭示了粪肠球菌在 PCP 冲击下的显著代谢变化，PCP 浓度范围为 0.05 至 100mg/L，并且可以在 8 分钟内检测到 0.05mg/L 的 PCP 对粪肠球菌的毒性。此外，通过将该技术扩展到混合厌氧污泥中，不仅可以在 10 分钟内确定 0.05-100mg/L PCP 对厌氧消化代谢的影响，还可以确定其沼气产生速率。这些结果表明，基于 Resazurin 的荧光测量法有可能被纳入微流控系统，以开发用于实时监测、控制和预警进入厌氧消化系统的毒物/抑制剂负荷的生物传感器。
