Kim Ju Il, Kwon Min, Lee Si Hyeock, Kim Yonggyun
Highland Agriculture Research Institute, Rural Development Administration, National Institute of Crop Science, Pyeong-chang 232-955, Republic of Korea.
Highland Agriculture Research Institute, Rural Development Administration, National Institute of Crop Science, Pyeong-chang 232-955, Republic of Korea.
Biochem Biophys Res Commun. 2015 Apr 17;459(4):579-84. doi: 10.1016/j.bbrc.2015.02.150. Epub 2015 Mar 11.
The genus Diadegma is a well-known parasitoid group and some are known to have symbiotic virus, polydnavirus (PDV). A novel IV was discovered from the calyx of Diadegma fenestrale female and sequenced its genome. D. fenestrale has more than two hosts, including potato tuber moth (PTM) and diamondback moth (DBM). D. fenestrale preferred PTM to DBM as hosts based on the oviposition and survival rate. Nevertheless, the developmental period and morphology of D. fenestrale were not significantly different between PTM and DBM. We compared DfIV gene expression patterns between PTM and DBM under various conditions to understand the phenomena. DfIV genes were more widely expressed in PTM with large numbers than in DBM after parasitized by D. fenestrale, particularly at the initial point. They showed differential expression patterns between two lepidopteran hosts. This DfIV gene expression plasticity showed a dependency on the lepidopteran host species and parasitization time, suggesting that it may contribute to increase the parasitoid survival rate. This might be one of the key elements that determine the symbiotic relationship between PDV and parasitoid.
迪亚德格玛属是一个著名的寄生蜂类群，已知其中一些寄生蜂携带共生病毒——多分DNA病毒（PDV）。从窗孔迪亚德格玛雌蜂的萼囊中发现了一种新型的IV，并对其基因组进行了测序。窗孔迪亚德格玛有两个以上的寄主，包括马铃薯块茎蛾（PTM）和小菜蛾（DBM）。基于产卵率和存活率，窗孔迪亚德格玛更喜欢将PTM作为寄主而非DBM。然而，窗孔迪亚德格玛在PTM和DBM上的发育时期和形态并没有显著差异。我们比较了在各种条件下PTM和DBM之间的DfIV基因表达模式，以了解这些现象。被窗孔迪亚德格玛寄生后，DfIV基因在PTM中的表达比在DBM中更广泛，数量也更多，尤其是在初始阶段。它们在两种鳞翅目寄主之间表现出差异表达模式。这种DfIV基因表达可塑性显示出对鳞翅目寄主物种和寄生时间的依赖性，表明它可能有助于提高寄生蜂的存活率。这可能是决定PDV与寄生蜂共生关系的关键因素之一。
