Hashimoto Makoto, Wakana Daigo, Ueda Miki, Kobayashi Daisuke, Goda Yukihiro, Fujii Isao
School of Pharmacy, Iwate Medical University, 2-1-1 Nishitokuta, Yahaba, Iwate 028-3694, Japan.
National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
Bioorg Med Chem Lett. 2015 Apr 1;25(7):1381-4. doi: 10.1016/j.bmcl.2015.02.057. Epub 2015 Feb 28.
Talaromyces stipitatus ATCC 10500 possesses 17 non-reducing polyketide synthase (NR-PKS) genes. During the course of our functional analysis of PKS genes with a C-terminus methyltransferase domain from T. stipitatus, we expressed tspks2, tspks3 and tspks4 genes in the heterologous host Aspergillus oryzae, respectively. Although the tspks4 transformant gave no apparent product in HPLC analysis, a novel azaphilone pentaketide (3) was identified along with two known related products from the tspks2 transformant. Of four hexaketide products from the tspks3 transformant, two new compounds were identified to be 2-acetyl-7-methyl-3,6,8-trihydroxynaphthalene (4) and its derivative fused with α-methyl-α,β-unsaturated γ-lactone (7).
柄孢壳菌ATCC 10500拥有17个非还原型聚酮合酶（NR-PKS）基因。在我们对柄孢壳菌中具有C端甲基转移酶结构域的PKS基因进行功能分析的过程中，我们分别在异源宿主米曲霉中表达了tspks2、tspks3和tspks4基因。尽管tspks4转化体在HPLC分析中未产生明显产物，但从tspks2转化体中鉴定出了一种新的氮杂蒽酮五酮（3）以及两种已知的相关产物。在tspks3转化体的四种六酮产物中，鉴定出两种新化合物为2-乙酰基-7-甲基-3,6,8-三羟基萘（4）及其与α-甲基-α,β-不饱和γ-内酯融合的衍生物（7）。
