2009-2011 年期间科威特住院患者中检测到的人类偏肺病毒的系统进化分析。

Phylogenetic analysis of human metapneumovirus detected in hospitalized patients in Kuwait during the years 2009-2011.

机构信息

Department of Microbiology, Faculty of Medicine, Kuwait University, Jabriya, Kuwait.

出版信息

J Infect Public Health. 2015 Sep-Oct;8(5):448-57. doi: 10.1016/j.jiph.2015.01.008. Epub 2015 Mar 12.

DOI:10.1016/j.jiph.2015.01.008

PMID:25773766

Abstract

BACKGROUND

Human metapneumovirus (hMPV) is an important cause of both upper and lower respiratory tract infections (RTIs) in all age groups. Children, elderly, and immunocompromised individuals are the most affected groups. HMPV infection accounts for 5% of hospitalized patients with respiratory tract infections in Kuwait. It is mostly detected among infants and elderly age groups, and both hMPV genotypes A and B circulate in Kuwait.

METHODS

In this study, the genetic diversity of detected hMPV was evaluated, and a phylogenetic analysis based on partial nucleotide and amino acid sequences of the G gene was performed for hMPV detected among hospitalized patients with RTIs.

RESULTS

Our results showed that 62% of hMPV sequences belonged to the A genotype and 38% to the B genotype. A2b and B2 subtypes were detected and circulated during the study period, whereas A1 and B1 subtypes were not detected. Based on nucleotide sequences of the G gene, most of hMPV strains (57%) were clustered with Indian strains, followed by Greek strains (24%) and Canadian strains (14%). One strain (5%) clustered within the B genotype but had different branches than B1 and B2 branches.

CONCLUSION

Our data showed the co-circulation of hMPV genotypes A2b and B2 in Kuwait with genetic diversity suggestive of evolution through negative selection.

摘要

背景

人偏肺病毒（hMPV）是所有年龄段人群上呼吸道和下呼吸道感染（RTIs）的重要病因。儿童、老年人和免疫功能低下者是受影响最严重的群体。在科威特，hMPV 感染占呼吸道感染住院患者的 5%。它主要在婴儿和老年人群中检测到，并且在科威特同时流行 hMPV 基因型 A 和 B。

方法

在这项研究中，评估了检测到的 hMPV 的遗传多样性，并对住院 RTIs 患者中检测到的 hMPV 的 G 基因部分核苷酸和氨基酸序列进行了基于系统进化的分析。

结果

我们的结果表明，62%的 hMPV 序列属于基因型 A，38%属于基因型 B。在研究期间检测到并流行 A2b 和 B2 亚型，而未检测到 A1 和 B1 亚型。基于 G 基因的核苷酸序列，大多数 hMPV 株（57%）与印度株聚类，其次是希腊株（24%）和加拿大株（14%）。一个株（5%）聚类在 B 基因型内，但与 B1 和 B2 分支不同。