核糖体RNA修饰在蛋白质合成和核糖体组装中的作用的结构见解。
Structural insights into the role of rRNA modifications in protein synthesis and ribosome assembly.
作者信息
Polikanov Yury S, Melnikov Sergey V, Söll Dieter, Steitz Thomas A
机构信息
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, USA.
Howard Hughes Medical Institute at Yale University, New Haven, CT, USA.
出版信息
Nat Struct Mol Biol. 2015 Apr;22(4):342-344. doi: 10.1038/nsmb.2992. Epub 2015 Mar 16.
We report crystal structures of the Thermus thermophilus ribosome at 2.3- to 2.5-Å resolution, which have enabled modeling of rRNA modifications. The structures reveal contacts of modified nucleotides with mRNA and tRNAs or protein pY, and contacts within the ribosome interior stabilizing the functional fold of rRNA. Our work provides a resource to explore the roles of rRNA modifications and yields a more comprehensive atomic model of a bacterial ribosome.
我们报告了嗜热栖热菌核糖体在2.3至2.5埃分辨率下的晶体结构，这些结构使得对rRNA修饰进行建模成为可能。这些结构揭示了修饰核苷酸与mRNA、tRNA或蛋白质pY的相互作用，以及核糖体内部稳定rRNA功能折叠的相互作用。我们的工作为探索rRNA修饰的作用提供了资源，并产生了一个更全面的细菌核糖体原子模型。
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