使用含有多个环糊精的三（联吡啶）钌（II）配合物对溶菌酶进行灵敏的荧光检测。

Sensitive fluorescence detection of lysozyme using a tris(bipyridine)ruthenium(II) complex containing multiple cyclodextrins.

作者信息

Zhang Fan, Zhao Ying-Ying, Chen Hong, Wang Xiu-Hua, Chen Qiong, He Pin-Gang

机构信息

Department of Chemistry, East China Normal University, No. 500 Dongchuan Road, Shanghai 200241, P. R. China.

出版信息

Chem Commun (Camb). 2015 Apr 18;51(30):6613-6. doi: 10.1039/c5cc00428d.

DOI:10.1039/c5cc00428d

PMID:25776220

Abstract

A new series of photoactive metallocyclodextrins with increased fluorescence intensity upon binding with ssDNAs/aptamers has been demonstrated to sensitively and selectively detect lysozyme. The detection mechanism relies on the formation of an aptamer-lysozyme complex, which leads to reduction of fluorescence intensity.

摘要

一系列新的光活性金属环糊精已被证明能在与单链DNA/适体结合时增加荧光强度，从而灵敏且选择性地检测溶菌酶。检测机制依赖于适体-溶菌酶复合物的形成，这会导致荧光强度降低。