Li Xin, Bi Yang, Wang Junjie, Dong Boyu, Li Haijie, Gong Di, Zhao Ying, Tang Yamei, Yu Xiaoyan, Shang Qi
College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China.
College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China.
J Proteomics. 2015 Apr 29;120:179-93. doi: 10.1016/j.jprot.2015.03.006. Epub 2015 Mar 14.
Benzo-(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester (BTH) is a chemical plant elicitor capable of inducing disease resistance in many crops. In this study, the climacteric fruit muskmelon (cv. Yujinxiang) was treated with BTH at 0.1g/L for assaying the changes in physiology, biochemistry and protein profile during ripening. The results showed that BTH treatment enhanced respiration rate, while reduced titratable acid content and retarded the decline of fruit firmness and ascorbic acid content. Ethylene production increased after BTH treatment at early stages of ripening, but decreased after 6days of treatment. Of the detected protein spots separated by means of 2-DE, 69 spots changed in abundance significantly after BTH treatment. Fifty-two spots out of 69 were identified using MALDI-TOF/TOF by blasting against NCBInr database. Functional classification revealed that the protein species identified were related to defense and stress responses, protein synthesis, destination and storage, energy metabolism, primary metabolism, cell structure, secondary metabolism, signal transduction and transporters. This study demonstrates an overview of major physiological, biochemical and proteomic changes in muskmelon fruit during ripening after BTH treatment and provides potentially useful information for maintaining fruit quality and delaying the ripening and senescence process.
The study offers new proteomic evidences for elucidating the regulatory mechanism of muskmelon fruit ripening by BTH treatment at proteomic level, and provides a valuable reference for further research on the relationship between fruit quality and induction disease resistance in BTH-treated fruits.
苯并 -（1,2,3）-噻二唑 -7-硫代羧酸 S-甲酯（BTH）是一种能够在许多作物中诱导抗病性的化学植物激发子。在本研究中，用 0.1g/L 的 BTH 处理跃变型果实甜瓜（品种：玉金香），以测定其成熟过程中的生理、生化和蛋白质谱变化。结果表明，BTH 处理提高了呼吸速率，同时降低了可滴定酸含量，并延缓了果实硬度和抗坏血酸含量的下降。在成熟早期，BTH 处理后乙烯产量增加，但处理 6 天后乙烯产量下降。通过二维电泳分离检测到的蛋白质斑点中，有 69 个斑点在 BTH 处理后丰度发生了显著变化。通过与 NCBInr 数据库比对，利用基质辅助激光解吸电离飞行时间串联质谱（MALDI-TOF/TOF）鉴定出 69 个斑点中的 52 个。功能分类显示，鉴定出的蛋白质种类与防御和应激反应、蛋白质合成、转运和储存、能量代谢、初级代谢、细胞结构、次生代谢、信号转导和转运蛋白有关。本研究概述了 BTH 处理后甜瓜果实成熟过程中的主要生理、生化和蛋白质组学变化，并为维持果实品质和延缓成熟衰老过程提供了潜在有用的信息。
该研究为从蛋白质组学水平阐明 BTH 处理对甜瓜果实成熟的调控机制提供了新的蛋白质组学证据，并为进一步研究 BTH 处理果实的品质与诱导抗病性之间的关系提供了有价值的参考。
