Luna A, Jimenez-Rios G, Villanueva E
Forensic Sci Int. 1985 Nov-Dec;29(3-4):171-8. doi: 10.1016/0379-0738(85)90110-0.
Brain autolysis happens rapidly, especially when environmental temperatures are high, and poses serious limitations for evaluating damage using morphologic methods. In the present study we have measured total proteins, cathepsin A and aminopeptidase activity in the vitreous humor in relation to cause of death and survival time. We have studied vitreous humor samples from 106 cadavers autopsied in the I.A.F. of Granada. The samples were classified according to causes of death as follows: myocardial infarction; hanging; other mechanical asphyxias; multiple trauma; craniocerebral trauma; other violent death; pulmonary functions; and other natural deaths. Total protein was measured by Lowry's method, and cathepsin A and aminopeptidase activity by the Bowen and Davison (1973) and Greenberg (1962) methods, respectively. Results are expressed in IU/l and in mIU/mg of protein. We found higher values of aminopeptidase and cathepsin A activity in groups with severe brain damage (craniocerebral trauma, multiple trauma, etc.) and lower values in groups of natural deaths. We believe, therefore, that aminopeptidase activity in vitreous humor may be a useful parameter for evaluating brain damage.
脑自溶发生迅速，尤其是在环境温度较高时，这给使用形态学方法评估损伤带来了严重限制。在本研究中，我们测量了玻璃体液中的总蛋白、组织蛋白酶A和氨肽酶活性，并将其与死因和存活时间相关联。我们研究了在格拉纳达法医研究所进行尸检的106具尸体的玻璃体液样本。样本根据死因分类如下：心肌梗死；缢死；其他机械性窒息；多处创伤；颅脑创伤；其他暴力死亡；肺功能障碍；以及其他自然死亡。总蛋白采用洛瑞法测定，组织蛋白酶A和氨肽酶活性分别采用鲍恩和戴维森（1973年）以及格林伯格（1962年）的方法测定。结果以国际单位/升和毫国际单位/毫克蛋白表示。我们发现，在脑损伤严重的组（颅脑创伤、多处创伤等）中，氨肽酶和组织蛋白酶A活性值较高，而在自然死亡组中值较低。因此，我们认为玻璃体液中的氨肽酶活性可能是评估脑损伤的一个有用参数。
