Shen Yan, Wang Jun, Liu Xuewu, Liang Jiao, Huang Yuxiao, Liu Zhongxiang, Zhao Y A, Li Yinghui
Department of Medical Microbiology and Parasitology, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China.
Exp Ther Med. 2015 Apr;9(4):1357-1362. doi: 10.3892/etm.2015.2237. Epub 2015 Jan 29.
There is great interest in any new discoveries in malaria vaccine research. reticulocyte-binding protein homolog 5 (PfRH5) shows promise in this area and may be used together with other merozoite antigens as a potential vaccine. In the present study, a bioinformatics prediction approach was applied to a PfRH5 B-cell epitope, and two B-cell epitope distributions were selected. Antibodies against the two PfRH5 distributions were obtained and the growth activity inhibition was measured. No inhibition of the CY strain was found, but the growth of the 3D7 strain was inhibited by all of the antibodies, in contrast to the results of other studies. It was additionally found that certain quantities of protein led to the inhibition of the parasitic invasion. Equally noteworthy was that the survival time of the group immunized with a portion of PfRH5 was significantly longer than that of the group immunized with the full-length protein, following infection by ANKA. The present study produced conflicting results in and experiments, although the accuracy of the evaluation may be lessened due to the use of a murine malaria model. The findings of the present study may indicate that PfRH5 may not be suitable in malaria vaccine research.
疟疾疫苗研究中的任何新发现都备受关注。网织红细胞结合蛋白同源物5（PfRH5）在该领域显示出前景，可能与其他裂殖子抗原一起用作潜在疫苗。在本研究中，将生物信息学预测方法应用于PfRH5 B细胞表位，并选择了两种B细胞表位分布。获得了针对这两种PfRH5分布的抗体，并测量了生长活性抑制情况。未发现对CY株有抑制作用，但与其他研究结果相反，所有抗体均抑制了3D7株的生长。还发现一定量的蛋白质导致寄生虫入侵受到抑制。同样值得注意的是，在用ANKA感染后，用部分PfRH5免疫的组的存活时间明显长于用全长蛋白质免疫的组。尽管由于使用小鼠疟疾模型可能会降低评估的准确性，但本研究在实验中产生了相互矛盾的结果。本研究结果可能表明PfRH5可能不适用于疟疾疫苗研究。
