Yeo In Ho, Lee Eun Yong
Department of Acupuncture & Moxibustion Medicine, Semyung University Oriental Medicine Hospital, Chungju, Korea.
J Pharmacopuncture. 2014 Jun;17(2):41-5. doi: 10.3831/KPI.2014.17.015.
This study was performed to analyze the single-dose toxicity of neutral natured blood stasis pharmacopuncture extracts.
All experiments were conducted at Biotoxtech, an institution authorized to perform non-clinical studies, under the regulations of Good Laboratory Practice (GLP). Sprague-Dawley rats were chosen for the pilot study. Doses of neutral natured blood stasis pharmacopuncture extracts, 0.1, 0.5 and 1.0 mL, were administered to the experimental group, and the same doses of normal saline solution were administered to the control group. This study was conducted under the approval of the Institutional Animal Ethics Committee.
In all 4 groups, no deaths occurred, and the neutral natured blood stasis pharmacopuncture extracts administered by intramuscular (IM) injection was over 1.0 mL/animal. No significant changes in the body weights between the control group and the experimental group were observed. To check for abnormalities in organs and tissues, we used microscopy to examine representative histological sections of each specified organ; the results showed no significant differences in any organs or tissues.
The above findings suggest that treatment with neutral natured blood stasis pharmacopuncture extracts is relatively safe. Further studies on this subject should be conducted to yield more concrete evidence.
本研究旨在分析中性血瘀型药针提取物的单剂量毒性。
所有实验均在Biotoxtech进行，该机构是一家获授权开展非临床研究的机构，遵循良好实验室规范（GLP）的规定。选用Sprague-Dawley大鼠进行预实验。向实验组注射0.1、0.5和1.0 mL的中性血瘀型药针提取物，向对照组注射相同剂量的生理盐水。本研究在机构动物伦理委员会的批准下进行。
在所有4组中，均未出现死亡情况，经肌肉注射给予的中性血瘀型药针提取物剂量超过1.0 mL/只动物。对照组和实验组之间的体重未观察到显著变化。为检查器官和组织是否存在异常，我们使用显微镜检查了每个指定器官的代表性组织切片；结果显示任何器官或组织均无显著差异。
上述结果表明，使用中性血瘀型药针提取物治疗相对安全。应针对该主题开展进一步研究以获得更确凿的证据。
