脱落酸对水稻幼苗耐碱性胁迫的引发效应

Priming effect of abscisic acid on alkaline stress tolerance in rice (Oryza sativa L.) seedlings.

作者信息

Wei Li-Xing, Lv Bing-Sheng, Wang Ming-Ming, Ma Hong-Yuan, Yang Hao-Yu, Liu Xiao-Long, Jiang Chang-Jie, Liang Zheng-Wei

机构信息

Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, Jilin, 130102, China; University of Chinese Academy of Sciences, Beijing, 100049, China.

Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, Jilin, 130102, China; Da'an Sodic Land Experiment Station, Da'an, Jilin, 131317, China.

出版信息

Plant Physiol Biochem. 2015 May;90:50-7. doi: 10.1016/j.plaphy.2015.03.002. Epub 2015 Mar 12.

DOI:10.1016/j.plaphy.2015.03.002

PMID:25780993

Abstract

Saline-alkaline stress is characterized by high salinity and high alkalinity (high pH); alkaline stress has been shown to be the primary factor inhibiting rice seedling growth. In this study, we investigated the potential priming effect of abscisic acid (ABA) on tolerance of rice seedlings to alkaline stress simulated by Na2CO3. Seedlings were pretreated with ABA at concentrations of 0 (control), 10, and 50 μM by root-drench for 24 h and then transferred to a Na2CO3 solution that did not contain ABA. Compared to control treatment, pretreatment with ABA substantially improved the survival rate of rice seedlings and increased biomass accumulation after 7 days under the alkaline condition. ABA application at 10 μM also alleviated the inhibitory effects of alkaline stress on the total root length and root surface area. Physiologically, ABA increased relative water content (RWC) and decreased cell membrane injury degree (MI) and Na(+)/K(+) ratios. In contrast, fluridone (an ABA biosynthesis inhibitor) decreased the RWC and increased MI in shoots under the alkaline conditions. These data suggest that ABA has a potent priming effect on the adaptive response to alkaline stress in rice and may be useful for improving rice growth in saline-alkaline paddy fields.

摘要

盐碱胁迫的特点是高盐度和高碱度（高pH值）；碱胁迫已被证明是抑制水稻幼苗生长的主要因素。在本研究中，我们研究了脱落酸（ABA）对水稻幼苗耐碳酸钠模拟碱胁迫的潜在引发效应。通过根部浇灌用浓度为0（对照）、10和50 μM的ABA预处理幼苗24小时，然后转移到不含ABA的碳酸钠溶液中。与对照处理相比，ABA预处理显著提高了水稻幼苗的存活率，并增加了碱性条件下7天后的生物量积累。施用10 μM的ABA也减轻了碱胁迫对总根长和根表面积的抑制作用。生理上，ABA增加了相对含水量（RWC），降低了细胞膜损伤程度（MI）和Na(+)/K(+)比率。相反，氟啶酮（一种ABA生物合成抑制剂）在碱性条件下降低了地上部的RWC并增加了MI。这些数据表明，ABA对水稻对碱胁迫的适应性反应具有强大的引发效应，可能有助于改善盐碱稻田中水稻的生长。