在大鼠肝细胞中，通过受体介导将Ep459-去唾液酸胎球蛋白偶联物引入溶酶体后自噬溶酶体的积累。
Accumulation of autolysosomes after receptor-mediated introduction of Ep459-asialofetuin conjugate into lysosomes in rat hepatocytes.
作者信息
Furuno K, Miwa N, Ishikawa T, Kato K
出版信息
Exp Cell Res. 1985 Jan;156(1):15-21. doi: 10.1016/0014-4827(85)90257-5.
Administration of Ep459-asialofetuin conjugate (Ep459-AF) and pepstatin-asialofetuin conjugate (Ps-AF) to rats effectively inhibited lysosomal BANA hydrolase and cathepsin D in the liver, respectively, at a very low dose. Ep459-AF treatment also led to an accumulation of autolysosomes in rat liver. There was a close correlation between the accumulation of autolysosomes and the inhibition of BANA hydrolase activity. However, as opposed to the inhibition of thiol proteases, the inhibition of cathespin D did not cause accumulation of autolysosomes in the rat liver. These results suggest that autophagy in rat hepatocytes is a common occurrence under normal physiological conditions and that thiol proteases are digestive enzymes essential for the autolysomes.
给大鼠注射Ep459-去唾液酸胎球蛋白缀合物（Ep459-AF）和胃蛋白酶抑制剂-去唾液酸胎球蛋白缀合物（Ps-AF），能分别在极低剂量下有效抑制大鼠肝脏中的溶酶体BANA水解酶和组织蛋白酶D。Ep459-AF处理还导致大鼠肝脏中自噬溶酶体的积累。自噬溶酶体的积累与BANA水解酶活性的抑制之间存在密切相关性。然而，与巯基蛋白酶的抑制情况相反，组织蛋白酶D的抑制并未导致大鼠肝脏中自噬溶酶体的积累。这些结果表明，大鼠肝细胞中的自噬在正常生理条件下是常见现象，并且巯基蛋白酶是自噬溶酶体必不可少的消化酶。
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