Kauffman H F, van der Heide S, van der Laan S, Hovenga H, Beaumont F, de Vries K
Int Arch Allergy Appl Immunol. 1985;76(2):168-73.
During cultivation of Aspergillus fumigatus a rapid liberation of IgE-binding components was found reaching maximum values during the logarithmic phase of growth (phase I). After a fall in IgE-binding titers during phase II, appearance of additional IgE-binding components was noted during the period of lysis of the microorganism (phase III). These latter allergenic components are different from the phase I IgE-binding components, as was shown by crossed-inhibition studies. The number of precipitating antigenic components was not related with the corresponding IgE-binding titers and showed an increase during all phases of growth. The rapid changes in both IgE- and IgG-binding properties and the discrepancies between precipitating properties and IgE binding are discussed in relation to standardization and quality control of aspergillus extracts.
在烟曲霉培养过程中，发现其IgE结合成分迅速释放，在生长对数期（第一阶段）达到最大值。在第二阶段IgE结合滴度下降后，在微生物裂解期（第三阶段）发现了额外的IgE结合成分。交叉抑制研究表明，这些后期的变应原成分与第一阶段的IgE结合成分不同。沉淀抗原成分的数量与相应的IgE结合滴度无关，且在生长的所有阶段均有增加。结合曲霉提取物的标准化和质量控制，讨论了IgE和IgG结合特性的快速变化以及沉淀特性与IgE结合之间的差异。
