De Gregorio P R, Juárez Tomás M S, Leccese Terraf M C, Nader-Macías M E F
Centro de Referencia para Lactobacilos (CERELA)-CONICET, San Miguel de Tucumán, Tucumán, Argentina.
J Appl Microbiol. 2015 Apr;118(4):1034-47. doi: 10.1111/jam.12739. Epub 2015 Jan 29.
To assess the preventive effect of different intravaginal (i.va.) doses of Lactobacillus reuteri CRL1324 against vaginal colonization by Group B Streptococcus (GBS) in a murine experimental model.
The major virulence factors of four vaginal GBS clinical isolates were determined to select the most virulent strain and set up a murine model of streptococcal vaginal colonization. Later, the effect of four and seven doses of 10(8) viable cells of Lact. reuteri CRL1324 i.va. administered, prior to the GBS challenge was studied. Seven doses of lactobacilli were able to significantly reduce the number of viable GBS cells, while four doses showed no preventive effect. Both doses reduced the leucocyte influx induced by GBS. Seven doses caused a slight increase in the Lact. reuteri CRL1324 vaginal colonization compared with four doses and reduced murine vaginal pH compared to control mice.
Lactobacillus reuteri CRL1324 evidenced a preventive effect on GBS vaginal colonization in an experimental mouse model.
Maternal GBS colonization is one of the most important risk factors for developing disease in newborns. Lactobacillus reuteri CRL1324 could be considered as a new biological agent to reduce infections caused by this micro-organism.
在小鼠实验模型中评估不同阴道内剂量的罗伊氏乳杆菌CRL1324对B族链球菌（GBS）阴道定植的预防作用。
测定了4株阴道GBS临床分离株的主要毒力因子，以选择最具毒力的菌株并建立链球菌阴道定植的小鼠模型。随后，研究了在GBS攻击前经阴道给予4剂和7剂10⁸个罗伊氏乳杆菌CRL1324活细胞的效果。7剂乳酸杆菌能够显著减少GBS活细胞数量，而4剂则无预防作用。两种剂量均减少了GBS诱导的白细胞流入。与4剂相比，7剂使罗伊氏乳杆菌CRL1324的阴道定植略有增加，且与对照小鼠相比降低了小鼠阴道pH值。
罗伊氏乳杆菌CRL1324在实验小鼠模型中对GBS阴道定植有预防作用。
产妇GBS定植是新生儿发病的最重要危险因素之一。罗伊氏乳杆菌CRL1324可被视为一种新型生物制剂，用于减少由这种微生物引起的感染。
