Pessah M, Salvat C, Amit N, Infante R
Biochem Biophys Res Commun. 1985 Jan 16;126(1):373-81. doi: 10.1016/0006-291x(85)90616-3.
Polyribosomes were isolated from the intestinal mucosa of fasted and fat-fed rats in the presence of ribonuclease inhibitors. Polyribosomes from fat-fed rats were larger and more efficient in incorporating radioactive aminoacids into proteins than those from fasted rats. Total RNA prepared by guanidine-HCl extraction, from the intestine of fasting and fat-fed rats were translated in vitro in a mRNA-dependent rabbit reticulocyte lysate system in the presence of 35S-methionine. Sodium dodecyl sulfate polyacrylamide gel electrophoresis and autoradiography of the synthesized peptides showed a relative increase in the radioactivity of some peptides of RNA from fat-fed animals and particularly a two fold increase in preapo-AIV indicating that the intestinal apo-AIV synthesis is under transcriptional regulation by the metabolic processes involved in fat transport, that is, triglyceride-rich lipoprotein production.
在存在核糖核酸酶抑制剂的情况下，从禁食和高脂喂养大鼠的肠黏膜中分离出多核糖体。与禁食大鼠的多核糖体相比，高脂喂养大鼠的多核糖体更大，并且在将放射性氨基酸掺入蛋白质方面更有效。通过盐酸胍提取法从禁食和高脂喂养大鼠的肠道中制备的总RNA，在35S-甲硫氨酸存在的情况下，于依赖mRNA的兔网织红细胞裂解物系统中进行体外翻译。合成肽的十二烷基硫酸钠聚丙烯酰胺凝胶电泳和放射自显影显示，高脂喂养动物RNA的某些肽的放射性相对增加，尤其是前载脂蛋白AIV增加了两倍，这表明肠道载脂蛋白AIV的合成受脂肪转运相关代谢过程（即富含甘油三酯的脂蛋白产生）的转录调控。
