Muneer Sowbiya, Ko Chung Ho, Soundararajan Prabhakaran, Manivnnan Abinaya, Park Yoo Gyeong, Jeong Byoung Ryong
Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju, 660-701, Korea.
Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, 660-701, Korea.
PLoS One. 2015 Mar 19;10(3):e0120899. doi: 10.1371/journal.pone.0120899. eCollection 2015.
Although grafting is broadly used in the production of crops, no information is available about the proteins involved in vascular connections between rootstock and scion. Similarly, proteome changes under the light intensities widely used for grafted seedlings are of practical use. The objective of this study was to determine the proteome of vascular connections using watermelon (Citrullus vulgaris Schrad.) 'Sambok Honey' and 'Speed' as the scion and bottle gourd (Lagenaria siceraria Stanld.) 'RS Dongjanggun' as the rootstock grown under different light intensities (25, 50, 75 and 100 μmol m-2 s-1). Our proteomic analysis revealed 24 and 27 differentially expressed proteins in 'Sambok Honey' and 'Speed', respectively, under different light intensities. The identified proteins were largely involved in ion binding, amino acid metabolism, transcriptional regulation and defense response. The enhancement of ion-binding, transcriptional regulation, amino acid metabolism, and defense response proteins suggests a strengthening of the connection between the rootstock and scion under high light intensity. Indeed, the accumulation of key enzymes in the biological processes described above appears to play an important role in the vascular connections of grafted seedlings. Moreover, it appears that 100 μmol m-2 s-1 results in better protein expression responses in grafted seedlings.
虽然嫁接在作物生产中被广泛应用，但关于砧木和接穗之间维管连接所涉及的蛋白质尚无相关信息。同样，在广泛用于嫁接幼苗的光照强度下蛋白质组的变化也具有实际用途。本研究的目的是利用西瓜（Citrullus vulgaris Schrad.）‘三保蜂蜜’和‘斯皮德’作为接穗，瓠瓜（Lagenaria siceraria Stanld.）‘RS东janggun’作为砧木，在不同光照强度（25、50、75和100 μmol m-2 s-1）下生长，来确定维管连接的蛋白质组。我们的蛋白质组分析显示，在不同光照强度下，‘三保蜂蜜’和‘斯皮德’中分别有24种和27种差异表达蛋白。鉴定出的蛋白质主要参与离子结合、氨基酸代谢、转录调控和防御反应。离子结合、转录调控、氨基酸代谢和防御反应蛋白的增强表明在高光强下砧木和接穗之间的连接得到加强。事实上，上述生物过程中关键酶的积累似乎在嫁接幼苗的维管连接中起重要作用。此外，100 μmol m-2 s-1似乎能使嫁接幼苗产生更好的蛋白质表达反应。
