Turk J Med Sci. 2015;45(1):118-28. doi: 10.3906/sag-1311-107.
BACKGROUND/AIM: To assess the prognostic value of Ki-67, p27, and p53 immunoreactivity in human gastric cancer.
A total of 84 patients with gastric cancer participated in our study. We categorized tumors as intestinal and diffuse types, with reference to Lauren's classification. Ki-67, p27, and p53 immunoreactivity were correlated with patient's age, tumor type, grade, lymph node status, extent of invasion, tumor-node-metastasis (TNM) stage, and survival.
Decreased expression of p27 (<20% positivity of cells) and increased p53 staining (>50% positivity of cells) were determined in 41 (48.8%) and 29 (36.9%) tumor specimens, respectively, and were connected with both the TNM stage (P = 0.007 and P = 0.039, respectively), and the extent of tumor invasion (P = 0.025 and P = 0.004, respectively). Kaplan-Meier methods showed a remarkable effect of reduced p27 expression on survival time (P = 0.003). In contrast, we observed no notable relationship between survival time and p53 or Ki-67 immunoreactivity (P = 0.372 and P = 0.401, respectively).
A decrease in p27 expression and overexpression of p53 or Ki-67 may cause advancing and metastatic illness in patients with gastric carcinoma. In addition, immunopathological identification of p27 may be helpful to define patients with gastric cancer who are at an increased risk of death.
背景/目的：评估 Ki-67、p27 和 p53 免疫反应在人胃癌中的预后价值。
本研究共纳入 84 例胃癌患者。我们根据 Lauren 分类将肿瘤分为肠型和弥漫型。Ki-67、p27 和 p53 免疫反应与患者年龄、肿瘤类型、分级、淋巴结状态、浸润程度、肿瘤-淋巴结-转移（TNM）分期和生存相关。
41 例（48.8%）和 29 例（36.9%）肿瘤标本中分别确定 p27 表达降低（细胞阳性率<20%）和 p53 染色增加（细胞阳性率>50%），与 TNM 分期（P=0.007 和 P=0.039）和肿瘤侵袭程度（P=0.025 和 P=0.004）均有关。Kaplan-Meier 方法显示 p27 表达降低对生存时间有显著影响（P=0.003）。相反，我们观察到 p53 或 Ki-67 免疫反应与生存时间之间没有显著关系（P=0.372 和 P=0.401）。
p27 表达降低和 p53 或 Ki-67 过表达可能导致胃癌患者疾病进展和转移。此外，p27 的免疫病理学鉴定可能有助于确定胃癌患者死亡风险增加的患者。
