Turk J Med Sci. 2015;45(1):221-4. doi: 10.3906/sag-1312-38.
BACKGROUND/AIM: An experimental study was performed to evaluate the effect of extracorporeal shock wave lithotripsy (ESWL) on the distribution of interstitial cells of Cajal (ICC) in rabbit renal pelvis and proximal ureter.
Six New Zealand rabbits were included. Right kidneys were exposed to a total of 3000 shock waves (14 kV) by using an electrohydraulic-type ESWL device. Right sides were allocated as the ESWL group (EG, n = 6) and left sides as the control group (CG, n = 6). Tissues were harvested on day 7. Tissues were examined histopathologically for the presence of edema, inflammation, congestion, hemorrhage, fibrosis, and vascularization. Mast cell tryptase and CD 117 (c-kit) staining was performed for ICC distribution.
Although increased tissue edema in renal pelvises and increased inflammation in ureters were observed in EG, no statistical difference was detected between groups (P > 0.05). In CG, positive CD117 staining was detected in 2 renal pelvises and ureters. None of the EG samples showed CD117 staining and no statistical difference was detected between groups (P > 0.05).
Rabbit does not appear to be a good model for investigating ICCs. ESWL may cause histopathological alterations in the renal pelvis and ureter. Since it has not been statistically proven, reduced contractility of the ureter after ESWL may not be attributed to altered distribution of ICCs in the renal pelvis and ureter.
背景/目的：进行了一项实验研究，以评估体外冲击波碎石术（ESWL）对兔肾盂和输尿管问质细胞 Cajal（ICC）分布的影响。
纳入 6 只新西兰兔。使用液电式 ESWL 装置对右侧肾脏共施加 3000 次冲击波（14kV）。右侧作为 ESWL 组（EG，n=6），左侧作为对照组（CG，n=6）。第 7 天采集组织。检查组织病理学是否存在水肿、炎症、充血、出血、纤维化和血管化。进行肥大细胞 tryptase 和 CD117（c-kit）染色以观察 ICC 分布。
尽管 EG 中肾盂组织水肿增加，输尿管炎症增加，但组间无统计学差异（P>0.05）。CG 中 2 个肾盂和输尿管均有阳性 CD117 染色。EG 样本均无 CD117 染色，组间无统计学差异（P>0.05）。
兔似乎不是研究 ICC 的理想模型。ESWL 可能导致肾盂和输尿管的组织病理学改变。由于尚未进行统计学证明，ESWL 后输尿管收缩性降低可能与肾盂和输尿管 ICC 分布改变无关。
