Salman A, Shufan E, Lapidot I, Tsror L, Moreh R, Mordechai S, Huleihel M
Department of Physics, SCE - Shamoon College of Engineering, Beer-Sheva 84100, Israel. ahmad@ sce.ac.il.
Analyst. 2015 May 7;140(9):3098-106. doi: 10.1039/c5an00213c. Epub 2015 Mar 20.
Colletotrichum coccodes (C. coccodes) is a pathogenic fungus that causes anthracnose on tomatoes and black dot disease in potatoes. It is considered as a seed tuber and soil-borne pathogen that is difficult to control. C. coccodes isolates are classified into Vegetative Compatibility Groups (VCGs). Early classification of isolates into VCGs is of great importance for a better understanding of the epidemiology of the disease and improving its control. Moreover, the differentiation among these isolates and the assignment of newly-discovered isolates enable control of the disease at its early stages. Distinguishing between isolates using microbiological or genetic methods is time-consuming and not readily available. Our results show that it is possible to assign the isolates into their VCGs and to classify them at the isolate level with a high success rate using Principal Component Analysis (PCA) and Linear Discriminant Analysis (LDA).
番茄炭疽病菌（Colletotrichum coccodes，简称C. coccodes）是一种致病真菌，可引发番茄炭疽病和马铃薯黑点病。它被视为一种种薯和土壤传播的病原体，难以防治。C. coccodes分离株被归类为营养亲和群（VCGs）。将分离株尽早归类到VCGs中，对于更好地了解该病害的流行病学及改进防治措施具有重要意义。此外，区分这些分离株并对新发现的分离株进行归类，有助于在病害早期进行控制。使用微生物学或遗传学方法区分分离株既耗时又不易实现。我们的结果表明，利用主成分分析（PCA）和线性判别分析（LDA），能够将分离株归类到其VCGs中，并在分离株水平上进行分类，成功率很高。
