Ghisi Alexandre Corrêa, Kopper Patrícia Maria Poli, Baldasso Flávia E R, Stürmer Caroline P, Rossi-Fedele Giampiero, Steier Liviu, de Figueiredo José Antonio Poli, Morgental Renata Dornelles, Vier-Pelisser Fabiana Vieira
Department of Endodontics, School of Dentistry, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
Department of Conservative Dentistry, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
J Endod. 2015 Jun;41(6):925-30. doi: 10.1016/j.joen.2015.01.039. Epub 2015 Mar 17.
This study aimed to evaluate the effects of Sterilox (Sx), a superoxidized water, 5% and 2% sodium hypochlorite (5NaOCl and 2NaOCl), and 17% EDTA (E) on the organic and inorganic components of bovine dentin.
Eighty bovine incisors were randomly divided into 8 groups (n = 10): 5NaOCl, 5NaOCl + E, 2NaOCl, 2NaOCl + E, Sx, Sx + E, E alone, and distilled water (H2O). Root canal instrumentation was performed by using the corresponding irrigant. The apical 15 mm was longitudinally sectioned into 2 fragments, one for light microscopy analysis in slides stained with picrosirius red (organic component) and the other for scanning electron microscopy analysis (inorganic component). Scores data obtained in the light microscopy analysis were submitted to the Kruskal-Wallis test, followed by multiple comparisons test (P < .05). Scanning electron microscopy images were analyzed descriptively.
The chemical solution 5NaOCl had a greater effect on the organic component of dentin in area and depth than 2NaOCl. The chemical solutions 5NaOCl + E, 5NaOCl and 2NaOCl + E caused the greatest change in the collagenous organic matrix near the root canal lumen. The chemical solution 2NaOCl showed similar behavior to Sx, associated or not with E, promoting more superficial disorganization of collagen in a smaller area. Demineralization was observed in all groups in which E was used. However, areas of erosion and open dentinal tubules were detected only when it was combined with NaOCl.
Five percent NaOCl promoted the most extensive damage to the organic component of dentin, and when associated to EDTA, dentinal erosion could be seen. Considering these specific aspects, 2% NaOCl and Sx had milder effects on bovine root dentin.
本研究旨在评估超氧化水Sterilox（Sx）、5%和2%的次氯酸钠（5NaOCl和2NaOCl）以及17%的乙二胺四乙酸（E）对牛牙本质有机和无机成分的影响。
80颗牛切牙随机分为8组（n = 10）：5NaOCl组、5NaOCl + E组、2NaOCl组、2NaOCl + E组、Sx组、Sx + E组、单独使用E组和蒸馏水（H2O）组。使用相应的冲洗剂进行根管预备。将根尖15 mm纵向切成2个片段，一个用于用苦味酸天狼星红染色的载玻片上的光学显微镜分析（有机成分），另一个用于扫描电子显微镜分析（无机成分）。光学显微镜分析获得的评分数据进行Kruskal-Wallis检验，随后进行多重比较检验（P <.05）。对扫描电子显微镜图像进行描述性分析。
化学溶液5NaOCl在面积和深度上对牙本质有机成分的影响比2NaOCl更大。化学溶液5NaOCl + E、5NaOCl和2NaOCl + E在根管腔附近的胶原有机基质中引起的变化最大。化学溶液2NaOCl与Sx（无论是否与E联合使用）表现出相似的行为，在较小区域促进胶原更浅表的紊乱。在所有使用E的组中均观察到脱矿。然而，仅当E与NaOCl联合使用时才检测到侵蚀区域和开放的牙本质小管。
5%的NaOCl对牙本质有机成分造成的损伤最广泛，与EDTA联合使用时可观察到牙本质侵蚀。考虑到这些特定方面，2%的NaOCl和Sx对牛牙根牙本质的影响较温和。
