Bilvinaite Goda, Zongolaviciute Ruta, Drukteinis Saulius, Bukelskiene Virginija, Cotti Elisabetta
Institute of Dentistry, Faculty of Medicine, Vilnius University, Zalgirio 115, LT-08217 Vilnius, Lithuania.
Department of Biological Models, Life Sciences Center, Institute of Biochemistry, Vilnius University, LT-10257 Vilnius, Lithuania.
J Funct Biomater. 2022 Jul 15;13(3):95. doi: 10.3390/jfb13030095.
In the present study we evaluated the cytotoxicity of super-oxidized water on human gingival fibroblasts and its efficacy in debris and smear layer removal from root canal walls. Cultured gingival fibroblasts were exposed to super-oxidized water (Sterilox), which was diluted in Iscove’s modified Dulbecco’s medium (IMDM) at 30%, 40%, 50%, 60% and 70% concentrations. The control group was maintained in IMDM. The cell viability was evaluated by means of an MTT assay after incubation periods of 1 h, 2 h, 24 h and 48 h. Pathological cellular changes were also observed under fluorescence and phase contrast microscopes. The efficacy in debris and smear layer removal was evaluated in comparison to the conventional application of sodium hypochlorite (NaOCl) and ethylenediaminetetraacetic acid (EDTA). Forty maxillary premolars were randomly divided into two equal groups (n = 20) and shaped with ProTaper NEXT rotary instruments using Sterilox or NaOCl/EDTA for irrigation. Afterwards, roots were split longitudinally and examined under a scanning electron microscope. The results revealed that super-oxidized water and sterile distilled water have acceptable biological properties for endodontic applications at concentrations up to 50% (p > 0.05). Moreover, super-oxidized water is equally effective in debris and smear layer removal as compared to NaOCl/EDTA (p > 0.05).
在本研究中，我们评估了超氧化水对人牙龈成纤维细胞的细胞毒性及其从根管壁去除碎屑和玷污层的效果。将培养的牙龈成纤维细胞暴露于超氧化水（Sterilox）中，该超氧化水在伊斯科夫改良的杜尔贝科培养基（IMDM）中分别以30%、40%、50%、60%和70%的浓度进行稀释。对照组维持在IMDM中。在孵育1小时、2小时、24小时和48小时后，通过MTT试验评估细胞活力。还在荧光显微镜和相差显微镜下观察细胞的病理变化。与传统应用次氯酸钠（NaOCl）和乙二胺四乙酸（EDTA）相比，评估了超氧化水在去除碎屑和玷污层方面的效果。将40颗上颌前磨牙随机分为两组，每组20颗，使用Sterilox或NaOCl/EDTA进行冲洗，并用ProTaper NEXT旋转器械进行根管预备。之后，将牙根纵向劈开，在扫描电子显微镜下检查。结果显示，浓度高达50%时，超氧化水和无菌蒸馏水在牙髓治疗应用中具有可接受的生物学特性（p>0.05）。此外，与NaOCl/EDTA相比，超氧化水在去除碎屑和玷污层方面同样有效（p>0.05）。
