Koj A, Gauldie J, Sweeney G D, Regoeczi E, Sauder D N
J Immunol Methods. 1985 Feb 11;76(2):317-27. doi: 10.1016/0022-1759(85)90309-6.
Cytokines released from monocytes upon stimulation by lipopolysaccharide cause a number of cells to undergo proliferative and synthetic changes. At least one of these cytokines affects hepatocytes in vivo causing increased synthesis of a series of acute-phase proteins. We have established an in vitro micro-assay for hepatocyte stimulating factor (HSF) using primary cultures of normal rat hepatocytes. Measurement of increased synthesis of alpha 2-macroglobulin and decreased synthesis of albumin caused by exogenously added factor constitute a sensitive parameter for quantifying HSF. For comparing various cytokines preparations, we have defined a unit of HSF activity in terms of a stimulation index. We have used this assay to follow some preliminary attempts to isolate the factors responsible for stimulation of synthesis of acute-phase reactant by the liver.
脂多糖刺激单核细胞释放的细胞因子会使许多细胞发生增殖和合成变化。这些细胞因子中至少有一种在体内会影响肝细胞，导致一系列急性期蛋白的合成增加。我们利用正常大鼠肝细胞的原代培养物建立了一种肝细胞刺激因子（HSF）的体外微量测定法。测量外源性添加因子引起的α2-巨球蛋白合成增加和白蛋白合成减少，构成了定量HSF的一个敏感参数。为了比较各种细胞因子制剂，我们根据刺激指数定义了一个HSF活性单位。我们利用这个测定法跟踪了一些初步尝试，以分离负责刺激肝脏合成急性期反应物的因子。
