Postgraduate Studies in Health Sciences, Faculty of Medicine, Federal University of Mato Grosso (UFMT), 78060-900 Cuiabá, MT, Brazil.
Postgraduate Studies in Health Sciences, Faculty of Medicine, Federal University of Mato Grosso (UFMT), 78060-900 Cuiabá, MT, Brazil; Department of Basic Health Sciences, Faculty of Medicine, Federal University of Mato Grosso (UFMT), 78060-900 Cuiabá, MT, Brazil.
J Ethnopharmacol. 2015 May 26;166:176-84. doi: 10.1016/j.jep.2015.03.021. Epub 2015 Mar 16.
Helicteres sacarolha A. St.- Hil. et al. popularly known in Brazil as 'semente-de-macaco', is widely employed in the popular medicine in many of parts of Brazil in the alleviation of symptoms of ailments such as peptic ulcer and inflammation. Up to the present, there is no study addressing the gastroprotective activity of the hydroethanolic extract of H. sacarolha and its possible mechanism of actions.
The hydroethanolic (70%) extract of H. sacarolha (HEHs) was obtained by maceration. The gastroprotective activity was assessed using gastric ulcer models induced by acidified ethanol, piroxicam, and water restraint stress in mice and rats at doses of 20, 50 and 250mg/kg p.o. Mechanistic studies involved the antisecretory assay evaluated with pylorus ligation in rats and pre-treatments with appropriate antagonists/inhibitors such as yohimbine, glibenclamide, indomethacin and l-NAME, effect on catalase and myeloperoxidase activities and gastric mucus determination using acidified ethanol- induced ulcer in mice.
HEHs at all doses tested demonstrated potent gastroprotective activities in the acute ulcer models. The gastroprotective activity of HEHs was attenuated by pre-treatments with yohimbine, glibenclamide, indomethacin and l-NAME. HEHs effectively reduced basal gastric juice production without any effect on the free and total acidity. The gastroprotective action of HEHs involved increasing the antioxidant enzyme catalase and mucus secretion and inhibition of neutrophyl infiltration as reflected by the reduction in the myeloperoxidase activity.
The results of this study gave a scientific support for the popular use of the leaves of H. sacarolha in the treatment of gastric ulcers and that it has a multi-targeted action.
巴西广泛使用 Helicteres sacarolha A. St.-Hil. 等植物的种子来治疗消化性溃疡和炎症等疾病的症状，这种植物俗称“猴面包树的种子”。迄今为止，还没有研究报道 Helicteres sacarolha 水醇提取物的胃保护活性及其可能的作用机制。
采用浸渍法从 Helicteres sacarolha 叶子中获得水醇（70%）提取物（HEHs）。通过酸化乙醇、吡罗昔康和水束缚应激诱导的胃溃疡模型，在 20、50 和 250mg/kg 体重灌胃给药，评估 HEHs 的胃保护活性。机制研究涉及通过结扎幽门在大鼠中评估的抗分泌作用，以及用适当的拮抗剂/抑制剂（如育亨宾、格列本脲、吲哚美辛和 l-NAME）预处理，在酸化乙醇诱导的胃溃疡模型中评估对过氧化氢酶和髓过氧化物酶活性以及胃粘液的影响。
在所有测试剂量下，HEHs 在急性溃疡模型中均表现出强大的胃保护活性。HEHs 的胃保护活性在预先用育亨宾、格列本脲、吲哚美辛和 l-NAME 预处理后减弱。HEHs 可有效减少基础胃液分泌，而对游离和总酸度无影响。HEHs 的胃保护作用涉及增加抗氧化酶过氧化氢酶和粘液分泌，并抑制中性粒细胞浸润，反映在髓过氧化物酶活性的降低。
本研究结果为 Helicteres sacarolha 叶子治疗胃溃疡的民间用途提供了科学依据，表明其具有多靶点作用。
