Li Zhao-Qun, Zhang Shuai, Luo Jun-Yu, Wang Chun-Yi, Lv Li-Min, Dong Shuang-Lin, Cui Jin-Jie
1] State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang 455000, China [2] College of Plant Protection, Nanjing Agricultural University/Key Laboratory of Integrated Management of Crop Diseases and Pests (Nanjing Agricultural University), Ministry of Education, Nanjing 210095, China.
State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang 455000, China.
Sci Rep. 2015 Mar 20;5:9324. doi: 10.1038/srep09324.
Differences in sex pheromone component can lead to reproductive isolation. The sibling noctuid species, Helicoverpa armigera and Helicoverpa assulta, share the same two sex pheromone components, Z9-16:Ald and Z11-16:Ald, but in opposite ratios, providing an typical example of such reproductive isolation. To investigate how the ratios of the pheromone components are differently regulated in the two species, we sequenced cDNA libraries from the pheromone glands of H. armigera and H. assulta. After assembly and annotation, we identified 108 and 93 transcripts putatively involved in pheromone biosynthesis, transport, and degradation in H. armigera and H. assulta, respectively. Semi-quantitative RT-PCR, qRT-PCR, phylogenetic, and mRNA abundance analyses suggested that some of these transcripts involved in the sex pheromone biosynthesis pathways perform. Based on these results, we postulate that the regulation of desaturases, KPSE and LPAQ, might be key factor regulating the opposite component ratios in the two sibling moths. In addition, our study has yielded large-scale sequence information for further studies and can be used to identify potential targets for the bio-control of these species by disrupting their sexual communication.
性信息素成分的差异会导致生殖隔离。棉铃虫和烟青虫这两种近缘夜蛾科物种，共享相同的两种性信息素成分，即Z9-16:醛和Z11-16:醛，但比例相反，这为此类生殖隔离提供了一个典型例子。为了研究这两种物种中性信息素成分的比例是如何受到不同调控的，我们对棉铃虫和烟青虫性信息素腺体的cDNA文库进行了测序。经过组装和注释后，我们分别在棉铃虫和烟青虫中鉴定出108个和93个可能参与性信息素生物合成、运输和降解的转录本。半定量RT-PCR、qRT-PCR、系统发育分析和mRNA丰度分析表明，这些参与性信息素生物合成途径的转录本发挥了作用。基于这些结果，我们推测去饱和酶、KPSE和LPAQ的调控可能是调节这两种近缘蛾类相反成分比例的关键因素。此外，我们的研究产生了大规模的序列信息以供进一步研究，并可用于通过干扰它们的性通讯来确定这些物种生物防治的潜在靶点。
