Department of Entomology, Iowa State University, Ames, Iowa, United States of America.
The Volcani Center, Institute of Plant Protection, ARO, Bet-Dagan, Israel.
PLoS One. 2019 Jul 22;14(7):e0220187. doi: 10.1371/journal.pone.0220187. eCollection 2019.
The pink bollworm, Pectinophora gossypiella, is a world-wide pest of cotton and in some parts of the cotton growing region is controlled by the mating disruption technique using synthetic sex pheromone. The sex pheromone consists of two compounds, (Z,Z)- and (Z,E)-7,11-hexadecadienyl acetates, in about a 50:50 ratio. However, recently, a population with sex pheromone compound ratios of about 62:38 were found in cotton fields that use mating disruption in Israel. To investigate how the change developed, we compared the pheromone gland transcriptomes between a reference laboratory population and a population obtained from an Israeli cotton field utilizing mating disruption. We analyzed four biological replicates from each population and found transcripts encoding 17 desaturases, 8 reductases, and 17 candidate acetyltransferases in both populations, which could be involved in sex pheromone biosynthesis. The expression abundance of some genes between the two populations was different. Some desaturases and candidate acetyltransferases were found to have mutated in one of the populations. The differentially expressed genes play potential roles in sex pheromone biosynthesis and could be involved in causing altered female sex pheromone ratios in the field population.
红铃虫，棉铃虫，是一种世界性的棉花害虫，在棉花种植区的某些地方，通过使用合成性信息素进行交配干扰来控制。性信息素由两种化合物组成，（Z，Z）-和（Z，E）-7,11-十六碳二烯基乙酸酯，比例约为 50:50。然而，最近在以色列使用交配干扰的棉田中发现了一种性信息素化合物比例约为 62:38 的种群。为了研究这种变化是如何发展的，我们比较了参考实验室种群和从利用交配干扰的以色列棉田获得的种群的信息素腺转录组。我们分析了每个种群的四个生物学重复，在两个种群中发现了编码 17 种去饱和酶、8 种还原酶和 17 种候选乙酰转移酶的转录本，这些酶可能参与性信息素生物合成。两个种群之间的一些基因的表达丰度不同。在一个种群中发现了一些去饱和酶和候选乙酰转移酶发生了突变。差异表达的基因在性信息素生物合成中发挥潜在作用，并可能参与导致田间种群中雌性性信息素比例发生改变。
