ICORP, Japan Science and Technology Agency (JST), The University of Tokyo, Tokyo, Japan.
ICORP, Japan Science and Technology Agency (JST), The University of Tokyo, Tokyo, Japan ; Department of Life Sciences (Biology), Graduate School of Arts and Sciences, The University of Tokyo, Tokyo, Japan ; Recipient of Imperial Prize and Japan Academy Prize in 2001.
Proc Jpn Acad Ser B Phys Biol Sci. 2006 Nov;82(7):197-207. doi: 10.2183/pjab.82.197. Epub 2006 Nov 18.
Mesoderm induction as a result of the interaction between endoderm and ectoderm is one of the most crucial events in vertebrate development. We identified activin as a strong mesoderm-inducing factor in an animal cap assay, an in vitro assay system using amphibian pluripotential cell mass. Activin induces mesodermal tisswes including most dorsal mesodermal tissue, notochord (which has important roles in neural induction, somite segmentation, and endodermal organogenesis), and its effects are concentration-dependent. Animal cap cells treated with high concentrations of activin differentiate into anterior endoderm, which can act as an organizer, or center of body patterning. We have established an in vitro induction system for 22 different organs and tissues using animal cap cells, and have isolated many organ-specific genes. With these useful methods, and analysis of newly isolated tissue- and organ-specific genes, the molecular biological "road map" for organogenesis is being established.
中胚层诱导是脊椎动物发育过程中最重要的事件之一，它是内胚层和外胚层相互作用的结果。我们在动物帽实验中鉴定出激活素是一种很强的中胚层诱导因子，动物帽实验是一种使用两栖类多潜能细胞团的体外实验系统。激活素诱导中胚层组织，包括大部分背侧中胚层组织、脊索（在神经诱导、体节分节和内胚层器官发生中具有重要作用），其作用具有浓度依赖性。用高浓度激活素处理的动物帽细胞分化为前内胚层，前内胚层可以作为组织者，或者说是身体形态发生的中心。我们已经使用动物帽细胞建立了 22 种不同器官和组织的体外诱导系统，并分离了许多器官特异性基因。利用这些有用的方法和对新分离的组织和器官特异性基因的分析，正在建立器官发生的分子生物学“路线图”。
