Mutebi John-Paul, Godsey Marvin, Smith Robert P, Renell Melanie R, Smith Leticia, Robinson Sara, Sears Stephen, Lubelczyk Charles
1 Centers for Disease Control and Prevention , Division of Vector-Borne Diseases, Fort Collins, Colorado.
Vector Borne Zoonotic Dis. 2015 Mar;15(3):210-4. doi: 10.1089/vbz.2014.1696.
During the fall of 2010, 332 deer serum samples were collected from 15 of the 16 (93.8%) Maine counties and screened for eastern equine encephalitis virus (EEEV) antibodies using plaque reduction neutralizing tests (PRNTs). The aim was to detect and map EEEV activity in the state of Maine. Forty-seven of the 332 (14.2%) sera were positive for EEEV antibodies, showing a much wider distribution of EEEV activity in Maine than previously known. The percentage of EEEV antibody-positive deer sera was ≥10% in six counties-Piscataquis (100%), Somerset (28.6%), Waldo (22.2%), Penobscot (21.7%), Kennebec (13.7%), and Sagadahoc (10%). Positive sera were detected in all the six counties (Somerset, Waldo, Penobscot, Kennebec, Cumberland, and York) that were positive in 2009, suggesting endemic EEEV activity in these counties. EEEV antibodies were not detected in sera collected in five counties-Franklin, Knox, Lincoln, Oxford, and Washington-which was either due to low sample size or lack of EEEV activity in these counties. Our data suggest higher EEEV activity in central Maine compared to southern Maine, whereas EEEV activity in Maine has historically been associated with the southern counties of York and Cumberland.
2010年秋季，从缅因州16个县中的15个县（93.8%）采集了332份鹿血清样本，并用蚀斑减少中和试验（PRNTs）检测东部马脑炎病毒（EEEV）抗体。目的是检测并绘制缅因州的EEEV活动情况。332份血清中有47份（14.2%）EEEV抗体呈阳性，表明缅因州的EEEV活动分布比之前所知的要广泛得多。在6个县中，EEEV抗体阳性鹿血清的比例≥10%，分别是皮斯卡塔quis县（100%）、萨默塞特县（28.6%）、沃尔多县（22.2%）、佩诺布斯科特县（21.7%）、肯纳贝克县（13.7%）和萨加达霍克县（10%）。在2009年呈阳性的所有6个县（萨默塞特县、沃尔多县、佩诺布斯科特县、肯纳贝克县、坎伯兰县和约克县）都检测到了阳性血清，表明这些县存在EEEV地方流行活动。在富兰克林县（Franklin）、诺克斯县（Knox）,林肯县（Lincoln）、牛津县（Oxford）和华盛顿县（Washington）采集的血清中未检测到EEEV抗体，这要么是因为样本量少，要么是因为这些县没有EEEV活动。我们的数据表明，缅因州中部的EEEV活动比南部更高，而缅因州的EEEV活动在历史上一直与约克县和坎伯兰县的南部有关。
