Quandt F N, Yeh J Z, Narahashi T
Neurosci Lett. 1985 Feb 28;54(1):77-83. doi: 10.1016/s0304-3940(85)80121-x.
The effects of tetrodotoxin on single Na+-channel currents recorded from excised patches of neuroblastoma cells were examined. Tetrodotoxin was found to cause a dose-dependent reduction in the frequency at which Na+ channels conduct during a series of depolarizations. Surviving conducting states had normal open times and current amplitudes. These effects could be explained by a model which includes initial binding of tetrodotoxin to a closed state of the channel with stable, complete block during the time the channel would normally be gated open.
研究了河豚毒素对从神经母细胞瘤细胞分离的膜片上记录的单个钠离子通道电流的影响。发现河豚毒素会导致在一系列去极化过程中钠离子通道导通频率出现剂量依赖性降低。存活的导通状态具有正常的开放时间和电流幅度。这些效应可以用一个模型来解释，该模型包括河豚毒素最初与通道的关闭状态结合，并在通道正常门控开放的时间内产生稳定、完全的阻断。
