Jin Hui, Liu Xifeng, Gui Rijun, Wang Zonghua
College of Chemical Science and Engineering, Collaborative Innovation Center for Marine Biomass Fiber, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, The State Key Laboratory Growing Base of Fiber Materials and Modern Textiles, Qingdao University, Shandong 266071, PR China.
Department of Orthopedic Surgery, Mayo Clinic, Rochester, MN 55905, USA.
Colloids Surf B Biointerfaces. 2015 Apr 1;128:498-505. doi: 10.1016/j.colsurfb.2015.02.049. Epub 2015 Mar 6.
We described a facile synthesis of pH and near-infrared (NIR) light dual-sensitive core/shell hybrid nanospheres, consisting of gold nanorods (GNR) as the core and poly(N-isopropylacrylamide-co-methacrylic acid) as the shell, p(NIPAM-MAA). The resultant GNR/p(NIPAM-MAA) nanospheres showed a core/shell structure, with an average diameter of ∼110nm and a strong longitudinal surface plasmon band at NIR region. Due to the photothermal effect of GNR and pH/thermal-sensitive volume transition of p(NIPAM-MAA) hydrogels, the nanospheres with loading of 5-fluorouracil (5-FU) by electrostatic interactions were developed as a smart carrier for pH- and photothermal-induced release of 5-FU. Experimental results testified that the cumulative release of 5-FU from nanospheres was markedly increased in a mild acidic medium. Moreover, a NIR light (808nm) irradiation triggered a greater and faster release of 5-FU, which was further testified by relevant results from in vitro cytotoxicity assay, in vivo tumor growth inhibition and histological images of ex vivo tumor sections. These results revealed significant applications of GNR/p(NIPAM-MAA) nanospheres in controlled release of anticancer agents and photothermal ablation therapy of tumor tissues, accompanied by synergistic effect of chem-photothermal therapy.
我们描述了一种简便的合成方法，用于制备pH和近红外（NIR）光双敏感的核/壳杂化纳米球，其由金纳米棒（GNR）作为核，聚（N-异丙基丙烯酰胺-共-甲基丙烯酸）作为壳，即p（NIPAM-MAA）。所得的GNR/p（NIPAM-MAA）纳米球呈现核/壳结构，平均直径约为110nm，在近红外区域有很强的纵向表面等离子体带。由于GNR的光热效应以及p（NIPAM-MAA）水凝胶的pH/热敏感体积转变，通过静电相互作用负载5-氟尿嘧啶（5-FU）的纳米球被开发为用于pH和光热诱导释放5-FU的智能载体。实验结果表明，在温和酸性介质中，纳米球中5-FU的累积释放量显著增加。此外，近红外光（808nm）照射引发了5-FU更大且更快的释放，体外细胞毒性测定、体内肿瘤生长抑制以及离体肿瘤切片的组织学图像的相关结果进一步证明了这一点。这些结果揭示了GNR/p（NIPAM-MAA）纳米球在抗癌药物控释和肿瘤组织光热消融治疗中的重要应用，并伴随着化学-光热疗法的协同效应。
