Muñoz-Esquerre Mariana, Huertas Daniel, Escobar Ignacio, López-Sánchez Marta, Penín Rosa, Peinado Victor, Barberà Joan Albert, Molina-Molina María, Manresa Frederic, Dorca Jordi, Santos Salud
Department of Pulmonary Medicine, Hospital Universitari de Bellvitge - IDIBELL, University of Barcelona, c/Feixa Llarga s/n. L'Hospitalet de Llobregat, CP 08907, Barcelona, Spain.
Lung. 2015 Jun;193(3):335-43. doi: 10.1007/s00408-015-9717-7. Epub 2015 Mar 21.
Fibronectin (Fn) and tenascin-C (TnC) are two extracellular matrix proteins associated with remodeling changes. Fn and TnC gene and protein expression in lung tissue, including their predominant location in bronchial and pulmonary artery structures, have not yet been fully evaluated. The aim of the present study was to assess: (1) gene expression of Fn and TnC in lung samples from chronic obstructive pulmonary disease (COPD) and non-COPD subjects; and (2) protein content and location of Fn and TnC in both groups.
Consecutive subjects requiring lung resection due to lung cancer surgery were included. Lung specimens were examined for gene expression by quantitative real-time PCR (values expressed as fold change ratio). The analysis of their protein content and location was performed by western blot and immunohistochemical studies, respectively. Patients were divided into two cohorts according to COPD status.
A total of 41 patients (20 with COPD and 21 without COPD) were included. An enhanced Fn gene expression was observed in the COPD group compared to the non-COPD group (4.73 ± 0.54 vs. 2.65 ± 0.57; P = 0.012), whereas no differences in gene TnC expression were observed (2.91 ± 0.44 vs. 2.60 ± 0.48; P = 0.633). No differences in lung protein content and location were found between groups. Immunohistochemical evaluation showed a predominantly vascular and bronchial location of Fn and TnC in both groups.
An enhanced lung gene expression of Fn was observed in COPD subjects compared to non-COPD subjects. No differences were found in Fn protein expression or in TnC gene or protein expression among groups.
纤连蛋白（Fn）和腱糖蛋白-C（TnC）是两种与重塑变化相关的细胞外基质蛋白。肺组织中Fn和TnC的基因及蛋白表达，包括它们在支气管和肺动脉结构中的主要定位，尚未得到充分评估。本研究的目的是评估：（1）慢性阻塞性肺疾病（COPD）患者和非COPD患者肺样本中Fn和TnC的基因表达；（2）两组中Fn和TnC的蛋白含量及定位。
纳入因肺癌手术需要肺切除的连续患者。通过定量实时聚合酶链反应检测肺标本的基因表达（数值以倍数变化率表示）。分别通过蛋白质印迹法和免疫组织化学研究分析其蛋白含量及定位。根据COPD状态将患者分为两个队列。
共纳入41例患者（20例COPD患者和21例非COPD患者）。与非COPD组相比，COPD组观察到Fn基因表达增强（4.73±0.54对2.65±0.57；P = 0.012），而TnC基因表达未观察到差异（2.91±0.44对2.60±0.48；P = 0.633）。两组之间肺蛋白含量及定位未发现差异。免疫组织化学评估显示两组中Fn和TnC主要定位于血管和支气管。
与非COPD患者相比，COPD患者肺组织中Fn基因表达增强。各组之间Fn蛋白表达、TnC基因或蛋白表达未发现差异。
