Feng Simin, Liu Songbai, Luo Zisheng, Tang Kaichen
Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310058, People's Republic of China.
Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310058, People's Republic of China; Fuli Institute of Food Science, Zhejiang University, Hangzhou 310058, People's Republic of China; Key Laboratory for Agro-Food Processing, Zhejiang University, Hangzhou 310058, People's Republic of China.
Food Chem. 2015 Aug 15;181:9-14. doi: 10.1016/j.foodchem.2015.02.073. Epub 2015 Feb 20.
A simple method based on direct saponification followed by RP-HPLC analysis was developed for quantification of free and conjugated sterols in sugarcane. Acid hydrolysis prior to alkaline saponification was used to determined acylated steryl glycoside and steryl glycoside in sugarcane. The applicability and generality of this method were improved with intensive investigation. Compared to traditional solvent extraction method, this method was more time saving and appropriate for characterization of sterol fractions in sugarcane. This method was successfully applied for determination of free and conjugated sterols in different sugarcane samples. The results exhibited that stigmasterol (varied from 883.3 ± 23.5 to 1823.9 ± 24.5 μg/g dry weigh) and β-sitosterol (varied from 117.6 ± 19.9 to 801.4 ± 33.5 μg/g dry weight) were major phytosterols in the sugarcane sample, and their glycosylated forms accounted for almost 87.0% of stigmasterol and 87.5% of β-sitosterol in sugarcane, respectively. In addition, among other parts of sugarcane, tips contained the greatest amount of phytosterols.
建立了一种基于直接皂化后进行反相高效液相色谱（RP-HPLC）分析的简单方法，用于定量甘蔗中的游离和共轭甾醇。在碱性皂化之前进行酸水解，以测定甘蔗中的酰化甾醇糖苷和甾醇糖苷。通过深入研究提高了该方法的适用性和通用性。与传统的溶剂萃取法相比，该方法更节省时间，适用于甘蔗中甾醇组分的表征。该方法成功应用于不同甘蔗样品中游离和共轭甾醇的测定。结果表明，豆甾醇（含量在883.3±23.5至1823.9±24.5μg/g干重之间）和β-谷甾醇（含量在117.6±19.9至801.4±33.5μg/g干重之间）是甘蔗样品中的主要植物甾醇，其糖基化形式分别占甘蔗中豆甾醇的近87.0%和β-谷甾醇的87.5%。此外，在甘蔗的其他部分中，梢部含有的植物甾醇量最大。
